摘要
目的研究黄芩甲苷对心肌缺血再灌注大鼠氧化应激反应的干预作用及其作用机制。方法将120只大鼠随机分为6组,每组20只:假手术组(sham组)、模型组、黄芩甲苷低(10mg/kg)、中(20mg/kg)、高(40mg/kg)剂量组,地尔硫(阳性对照药)组。采用夹闭冠状动脉的方法制备心肌缺血再灌注损伤大鼠模型,连续尾静脉治疗给药7d,给药结束后,于腹主动脉取血并分离得到血清,测定血清中谷草转氨酶(aspartateaminotransferase,AST)、磷酸肌酸激酶(phosphocreatinekinase,CPK)以及乳酸脱氢酶(lactatedehydrogenase,LDH)活性;取血结束后,每组随机取出大鼠心脏,切片,并进行2,3,5-氯化三苯基四氮唑(2,3,5-triphenyltetrazoliumchloride,TTC)染色,评估各组大鼠心肌梗死面积;每组另外10只取出心脏进行匀浆得心脏组织匀浆液,比色法检测心肌组织中氧化应激因子超氧化物歧化酶(superoxidedismutase,SOD)、还原性谷胱甘肽(Glutathione,GSH)、丙二醛(malondialdehyde,MDA)、过氧化氢酶(catalase,CAT)水平;Westernblot法检测心肌组织中血红素加氧酶1(hemeoxygenase1,HO-1)、新型核调节因子2(novelnuclearregulator2,Nrf2)蛋白表达并进行定量分析。结果黄芩甲苷低中高剂量组大鼠心肌组织梗死面积分别为(35.02±9.36)%,(29.41±6.38)%,(25.47±6.31)%;黄芩甲苷低剂量组血清中AST的含量为(477.00±83.49)kU/L,中剂量组血清中AST的含量为(398.00±64.17)kU/L,高剂量组中AST的含量为(408.00±48.38)kU/L;CPK的含量分别降为(523.00±49.36),(458±38.77),(336±36.13) kU/L;LDH的含量为(901.00±89.65),(867.00±84.04),(695±69.55)kU/L;心肌组织中SOD活性升高为(24.43±4.35),(27.14±3.18),(34.96±6.46)U/g;GSH的活性升高为(11.27±4.83),(14.70±2.49),(15.28±3.53)U/g;CAT活性升高为(7.08±0.96),(9.59±1.35),(12.51±2.36)U/g;MDA的活性降低为(28.97±4.46),(23.59±3.24),(16.04±2.67)U/g;同时对HO-1、Nrf2蛋白表达进行检测,HO-1蛋白相对表达量升高为(1.61±0.05),(1.73±0.03),(2.04±
Objective To study the effect and mechanism of astragaloside on oxidative stress induced by myocardial ischemia-reperfusion in rats.Methods Total of 120 rats were randomly divided into 6 groups with 20 rats in each group:sham operation group,model group,astragaloside(10,20,40 mg/kg)dose groups.Diltiazem(positive control drug)group.A rat model of myocardial ischemia-reperfusion injury was prepared by clamping the coronary arteries.Continuous tail vein treatment was given for 7 days.After administration,blood was collected from the abdominal aorta and serum was collected to determine serum aspartate aminotransferase(AST),creatine kinase(CPK)and lactate dehydrogenase(LDH)activity;At the end of blood collection,10 hearts in each group were randomly taken out and sectioned.TTC staining was performed to assess the myocardial infarct size of each group.10 hearts in each group were homogenized to obtain cardiac homogenate,and colorimetry was used to detect myocardial tissue.Oxidative stress factors such as superoxide dismutase(SOD),reduced glutathione(GSH),malondialdehyde(MDA),and catalase(CAT)levels were tested;HO-1 in myocardial tissue and Nrf2 protein expression were quantitative analysis by Western blot.Results The myocardial infarct area of rats in the low,middle and high dose group of baicalin were(35.02±9.36)%,(29.41±6.38)%,(25.47±6.31)%,respectively;The content of AST in serum of each group decreased to(477.00±83.49),(398.00±64.17),(408.00±48.38)kU/L;CPK content decreased to(523.00±49.36),(458.00±38.77),(336.00±36.13)kU/L;The content of LDH is(901.00±89.65),(867.00±84.04),(695±69.55)kU/L;The increase of SOD activity in myocardial tissue was(24.43±4.35),(27.14±3.18),(34.96±6.46)U/g;GSH activity increased to(11.27±4.83),(14.70±2.49),(15.28±3.53)U/g;CAT activity increased to(7.08±0.96),(9.59±1.35),and(12.51±2.36)U/g;The activity of MDA decreased to(28.97±4.46),(23.59±3.24),(16.04±2.67)U/g.At the same time,the expression of HO-1 and Nrf2 proteins were reduced.The relative expression of HO-1 pr
作者
鞠洪君
李庆辉
付光学
徐从健
岑丹
Ju Hongjun;Li Qinghui;Fu Guangxue;Xu Congjian;Cen Dan(People's Hospital of Guangrao County,Guangrao 257300,China;Department of Cardiology,Shengli Oilfield Central Hospital,Dongying 257000,China;Department of Gardiology,the Hospital of Sanhe City,Sanhe 065200,China)
出处
《国际免疫学杂志》
CAS
2020年第4期400-405,共6页
International Journal of Immunology
基金
三河市医学科技计划项目(2017SH03)。
关键词
黄芩甲苷
大鼠
心肌缺血再灌注
氧化应激
Astragalosides
Rat
Myocardial ischemia-reperfusion
Oxidative stress
