摘要
利用CRISPR/Cas9基因编辑技术依次敲除了野生型大肠杆菌MG1655的poxB、pta-ackA、ldhA、pflB、maeA、maeB和pfkA基因,最后获得突变菌株M07。摇瓶发酵实验结果表明,菌株M07发酵48 h时所得L-苹果酸产量达到9.893 g/L,得率为66%,相比野生型大肠杆菌MG1655相应值明显增加。
PoxB,pta-ackA,ldhA,pflB,maeA,maeB and pfkA genes of wild-type Escherichia coli MG1655 were removed in turn by using CRISPR/Cas9 gene editing technology,and finally the mutant strain M07 was obtained.The shaking flask fermentation experiment showed that when the strain M07 was fermented for 48 hours,the production and yield of L-malate reached 9.893 g/L and 66%,respectively,and the corresponding values were significantly increased than that of wild-type Escherichia coli MG1655.
作者
何彬
周志东
曹阳
黄皓
周卫
陈俊
He Bin;Zhou Zhidong;Cao Yang;Huang Hao;Zhou Wei;Chen Jun(College of Chemistry and Chemical Engineering,Wuhan University of Science and Technology,Wuhan 430081, China)
出处
《武汉科技大学学报》
CAS
北大核心
2020年第6期424-431,共8页
Journal of Wuhan University of Science and Technology
基金
国家自然科学基金资助项目(51608400)
湖北大学省部共建生物催化与酶工程国家重点实验室开放基金资助项目(SKLBEE2018002)
全国大学生创新创业大赛项目(201710488013).
