摘要
目的研究雪胆素甲(CUBα)对二甲基苯蒽(DMBA)诱导的小鼠乳腺肿瘤的影响。方法将实验小鼠按体质量采用配对设计随机分组法分为6组,每组12只。即空白对照组、模型对照组、他莫昔芬(阳性对照)组和CUBα小剂量组、中剂量组、大剂量组。建立DMBA诱导小鼠乳腺肿瘤模型,其中CUBα小剂量组、中剂量组、大剂量组小鼠分别灌胃给予CUBα12.5,25,50 mg·kg^-1,观察小鼠体温、体质量、瘤体生长及肺转移情况,测定小鼠白细胞计数水平、免疫脏器指数、血清雌二醇和孕酮含量,酶联免疫吸附法(ELISA)测定血浆肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)水平,检测组织中Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶、超氧化物歧化酶(SOD)、谷胱甘肽-过氧化物酶(GSH-Px)活性。结果与模型对照组比较,中、大剂量组CUBα乳腺肿瘤生长和肺转移结节数均被明显抑制,差异有统计学意义(P<0.05),雌二醇、孕酮含量降低,Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶、SOD、GSH-Px酶活性升高。与他莫昔芬组比较,大剂量CUBα组小鼠免疫指标显著改善,表现为白细胞计数水平和免疫器官脏器指数提高(均P<0.05),血浆TNF-α和IL-6水平降低(均P<0.05)。结论CUBα能抑制DMBA诱导的小鼠乳腺肿瘤,其作用机制与调剂免疫功能有关。
Objective To study the inhibitory effect of cucurbitacinα(CUBα)on methyl-nitrosourea(DMBA)-induced breast tumor growth in mice.Methods The mice were randomly divided into 6 groups according to their body weight,including blank control group,model control group,tamoxifen(positive control)group and CUBαlow-dose,medium dose and high-dose groups.DMBA induced breast tumor model was establshed in mice except blank control group.CUBαwas orally administered to mice at three doses[low(12.5 mg·kg^-1),middle(25 mg·kg^-1)and high dose(50 mg·kg^-1)].Temperature,body and tumor mass,and pulmonary metastasis were observed.WBC,Viscera index of thymus and spleen were measured.Serum levels of estrogen and progesterone were determinated.Plasma levels of TNF-αand IL-6 were assayed by ELISA.levels of WBC,TNF-α,IL-6 and Viscera index of thymus and spleen were measured.Activities of Na+-K+-ATP enzyme,Ca2+-Mg2+-ATP enzyme,superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were determined.Results Compared with model control group,CUBα(25 and 50 mg·kg^-1)could remarkably inhibit breast tumor growth and ameliorate the signs(P<0.05),reduce the level of estradiol and progesterone,protect the activities of SOD and GSH-Px,and suppress pulmonary metastasis rate.Compared with tamoxifen group,CUBα(50 mg·kg^-1)significantly improve the levels of WBC,TNF-αand IL-6,and Viscera index of thymus and spleen;and increased the activities of Na+-K+-ATP enzyme and Ca2+-Mg2+-ATP enzyme(P<0.05).Conclusion CUBαcould inhibit tumor growth and ameliorate the signs.The mechanism may be related to regulating the immune system.
作者
吴璐璐
田瑜
龚令熙
黄光英
WU Lulu;TIAN Yu;GONG Lingxi;HUANG Guangying(Department of Pharmacy, Second Affiliated Hospital of Army Medical University,Chongqing 400038,China)
出处
《医药导报》
CAS
北大核心
2020年第11期1471-1476,共6页
Herald of Medicine
关键词
雪胆素甲
乳腺肿瘤
免疫
Cucurbitacinα
Breast cancer
Immune
