摘要
探讨虾青素对2型糖尿病大鼠视网膜病变的保护作用及机制。将40只大鼠随机分为4组:正常组、模型组、低剂量实验组(虾青素15 mg/kg)、高剂量实验组(虾青素30 mg/kg),每组各10只。除正常组外,其它各组采用腹腔注射链脲佐菌素(STZ 30 mg/kg)并持续高糖高脂饲料饲养构建2型糖尿病大鼠模型;采用HE染色观察大鼠视网膜组织病理变化;免疫沉淀法检测大鼠视网膜组织中活性氧(ROS)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)水平;蛋白质免疫印迹法检测大鼠视网膜组织中色素上皮衍生因子(PEDF)和血管内皮生长因子(VEGF)水平。结果显示,与模型组比较,低、高剂量实验组大鼠视网膜组织中ROS、VEGF水平降低,CAT、SOD和PEDF水平升高,差异均有统计学意义(P<0.05)。高剂量实验组大鼠视网膜组织中CAT、SOD和PEDF水平高于低剂量实验组(P<0.05),ROS、VEGF水平低于低剂量实验组(P<0.05)。该结果表明虾青素对2型糖尿病大鼠视网膜病变有一定保护作用,其机制可能与调控氧化应激、PEDF和VEGF有关。
To explore the protective effect and mechanism of astaxanthin on retinopathy in type 2 diabetic rats.Forty rats were divided into 4 groups:normal group,model group,low-dose experimental group(astaxanthin 15 mg/kg),high-dose experimental group(astaxanthin 30 mg/kg),10 rats in each group.In addition to the normal group of rats,the other three groups were injected intraperitoneally with streptozotocin(STZ 30 mg/kg)and continuous feeding of high-sugar and high-fat feed were used to construct a type 2 diabetes rat model;HE staining was used to observe pathological changes of retinal tissue;immunoprecipitation method was used to detect reactive oxygen species(ROS),catalase(CAT)and superoxide dismutase(SOD)levels;western blot was used to detect pigment epithelium derived factor(PEDF)and vascular endothelial growth factor(VEGF)levels.The results showed that compared with the model group,the levels of ROS and VEGF decreased,and the levels of CAT,SOD,and PEDF increased in the low and high dose experimental groups(P<0.05).The levels of CAT,SOD and PEDF in the retinal tissue of the high-dose experimental group were higher than those of the low-dose experimental group(P<0.05),and the levels of ROS and VEGF were lower than those of the low-dose experimental group(P<0.05).The results indicate that astaxanthin has a certain protective effect on retinopathy in type 2 diabetic rats,and its mechanism may be related to the regulation of oxidative stress,PEDF and VEGF.
作者
杨倩
刘媛
李威威
YANG Qian;LIU Yuan;LI Weiwei(Department of the Third Ophthalmology,Baoding First Central Hospital,Baoding 071000,Hebei,China;Department of the Second Ophthalmology,Baoding First Central Hospital,Baoding 071000,Hebei,China)
出处
《中南医学科学杂志》
CAS
2020年第5期505-508,共4页
Medical Science Journal of Central South China
