摘要
以金钗石斛(Dendrobium nobide Lindl.)原球茎为材料,利用间歇浸没式植物生物反应器进行金钗石斛组培快繁的研究。通过优化浸没频率、接种量和培养基蔗糖浓度,获得最佳的培养条件为:浸没频率5 min/8 h、接种量10 g·L^-1、蔗糖浓度20 g·L^-1,并与传统的固体培养比较,反应器培养在组培苗的生长形态、增殖倍数和有效成分上都明显优于固体培养。利用间歇浸没式植物生物反应器进行金钗石斛种苗培养,为金钗石斛种苗的高效率、低成本的生产提供了一种新的方法。
Taking the protocorm of Dendrobium nobile Lindl.as material,the tissue culture and rapid propagation of D.nobile were studied in the temporary immersion bioreactor.The optimum culture conditions were obtained:the immersion frequency was 5 min/8 h,the inoculation amount was 10 g·L^-1 and the sucrose concentration was 20 g·L^-1.Compared with the traditional solid culture,the bioreactor culture was superior to the solid culture in the growth morphology,multiplication rate and effective components of the tissue culture seedlings,which provided a new method for the production of D.nobile seedlings with high efficiency and low cost.
作者
张杰
胡燕花
张本厚
陈集双
ZHANG Jie;HU Yanhua;ZHANG Benhou;CHEN Jishuang(Hangzhou Vocational&Technical College,Hangzhou 310018,China;Dafeng Marine Industrial Institute,Nanjing Tech University,Jiangsu Yancheng 224145,China;Institute of Bioresource Engineering,Nanjing Tech University,Nanjing 210009,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2020年第10期181-187,共7页
Journal of Agricultural Science and Technology
基金
国家自然科学基金项目(31560079)。
关键词
金钗石斛
植物组织培养
植物生物反应器
浸没频率
Dendrobium nobile Lindl.
plant tissue culture
plant bioreactor
immersion frequency
