摘要
目的探讨老年慢性阻塞性肺疾病(COPD)患者血清白介素(IL)-19、C1q肿瘤坏死因子相关蛋白(CTRP)-4水平表达及临床意义,为临床诊治提供理论依据。方法选取本院老年病科2017年1月至2019年6月诊治的156例COPD患者作为研究组,根据病情分为COPD急性加重期(89例,AECOPD组)和COPD稳定期(67例,COPD稳定期组);同时选取同期60名健康体检人群作为对照组。采用酶联免疫吸附试验法检测血清IL-19、CTRP-4水平,同时检测肺功能指标1 s用力呼气容积占用力肺活量百分比(FEV1/FVC)、1 s用力呼气容积占预计值的百分比(FEV1%pred)。应用Pearson相关性分析老年COPD患者IL-19、CTRP-4水平与肺功能指标的相关性;同时应用受试者工作曲线分析血清IL-19、CTRP-4水平对老年AECOPD患者的诊断价值。结果AECOPD组和COPD稳定期组血清IL-19、CTRP-4、CAT评分水平高于对照组(P<0.05),COPD稳定期组低于AECOPD(P<0.05)。AECOPD组和COPD稳定期组FEV1/FVC和FEV1%pred水平低于对照组(P<0.05),且COPD稳定期组高于AECOPD组(P<0.05)。Pearson相关性分析显示,COPD患者血清IL-19、CTRP-4水平与FEV1/FVC和FEV1%pred呈负相关(r=-0.504、-0.495;r=-0.534、-0.468,P均<0.05),与CAT评分呈正相关(r=0.441,0.517,P均<0.05)。经ROC曲线分析显示,IL-19、CTRP-4诊断AECOPD的曲线下面积分别为0.799、0.834,临界值为66.68 pg/mL、13.12μg/L时,灵敏度分别为75.28%、82.02%;特异性分别为3.53%、75.01%。结论COPD患者血清IL-19、CTRP-4水平明显上升,而且与肺功能和CAT评分存在相关性,可作为诊断AECOPD的标志物。
Objective To investigate the expressions and clinical significances of serum interleukin-19(IL-19)and tumor necrosis factor-4(CTRP-4)in elderly patients with chronic obstructive pulmonary disease(COPD),so as to improve the theoretical basis for clinical diagnosis and treatment.Methods From January,2017 to June,2019,156 patients with COPD diagnosed and treated were selected as the study group,who were divided into the acute exacerbation stage(89 cases,AECOPD group)and stable stage of COPD(67 cases,COPD stable stage group)according to their condition,and 60 healthy people were selected as the control group during the same period.The serum levels of IL-19 and CTRP-4 were measured by enzyme-linked immunosorbent assay(ELISA),and the pulmonary function indexes were also measured.The forced expiratory volume in one second accounted for the percentage of forced vital capacity(FEV1/FVC),and the forced expiratory volume in one second accounted for the percentage of predicted value(FEV1%pred)were tested.Pearson correlation method was used to analyze the correlation between the levels of IL-19,CTRP-4 and pulmonary function indexes in the elderly patients with COPD,and the diagnostic value of serum IL-19,CTRP-4 levels in the elderly patients with AECOPD was analyzed by the working curve of the subjects.Results The serum levels of IL-19,CTRP-4 and cat in the AECOPD group and COPD stable group were higher than those in the control group(P<0.05),and those in the COPD stable group were lower than the AECOPD group(P<0.05).FEV1/FVC and FEV1%pred levels in the AECOPD group and COPD stable period group were lower than those in the control group(P<0.05),and those in the COPD stable period group were higher than those in the AECOPD group(P<0.05).Pearson correlation analysis showed that the serum levels of IL-19 and CTRP-4 were negatively correlated with FEV1/FVC and FEV1%pred(r=-0.504,-0.495;r=-0.534,-0.468,P<0.05),while positively correlated with cat score(r=0.441,0.517,P<0.05).The ROC curve analysis showed that the area under the cur
作者
朱娅丽
张依
杜维桓
陈济超
张巍
李德善
纪红
ZHU Yali;ZHANG Yi;DU Weihuan;CHEN Jichao;ZHANG Wei;LI Deshan;JI Hong(Department of Geriatrics,Aerospace Center Hospital,Beijing 100049,China)
出处
《标记免疫分析与临床》
CAS
2020年第9期1460-1464,共5页
Labeled Immunoassays and Clinical Medicine
基金
吴阶平医学基金会临床科研专项资助基金(编号:320.6750.19089-2)。
