摘要
为探究某驴场幼驹腹泻暴发原因,采集腹泻驴驹病料进行细菌分离,对分离菌进行了菌落形态、PCR、生化特性、序列分析及药敏试验等鉴定。结果表明,2株分离菌为大肠杆菌,核苷酸序列同源性为98.9%。其耐药性强,对头孢氨苄等6种药物均有耐药性,对庆大霉素等4种药物中度敏感,仅对氧氟沙星等4种药物敏感,提示可选用敏感药物用于防治临床上的大肠杆菌感染,本试验为临床上驴驹腹泻防治提供了理论依据。
In order to explore the cause of diarrhea in a market,the diarrhea and sputum materials were collected for bacterial isolation,and the isolates were identified by colony morphology,PCR,biochemical characteristics,sequence analysis and drug susceptibility test.The results showed that the two isolates were E.coli and the nucleotide sequence homology was 98.9%.It had strong drug resistance.The isolates were resistant to 6 drugs such as cephalexin,and were moderately sensitive to 4 drugs such as gentamicin.It was sensitive only to 4 drugs such as ofloxacin,suggesting that sensitive drugs can be used.This study provides a theoretical basis for clinical prevention and treatment of diarrhea.
作者
冯培祥
杨莉
庄桂玉
赵付伟
嵇传良
王延涛
姜桂苗
FENG Pei-xiang;YANG Li;ZHUANG Gui-yu;ZHAO Fu-wei;JI Chuan-liang;WANG Yan-tao;JIANG Gui-miao(Dong-E E-Jiao Co.,Ltd.,National Engineering Research Center for Gelatin-based Traditional Chinese Medicine,Liaocheng 252200,China;Agricultural and Rural Bureau of Qingdao West Coast New Area,Qingdao 266400,China)
出处
《中国兽医杂志》
CAS
北大核心
2020年第6期90-92,97,共4页
Chinese Journal of Veterinary Medicine
关键词
大肠杆菌
分离鉴定
序列分析
药敏试验
E.coli
isolation and identification
sequence analysis
drug sensitivity test
