摘要
为了对商品肉鸡梭菌性肠炎的发病情况进行调查,本试验对收集的不同养殖模式的具有肠炎症状的肉鸡肠道内容物进行了产气荚膜梭菌的分离鉴定、活菌计数、菌株毒素类型鉴定以及动物试验,并对其中的43株分离株进行了药敏试验,同时也对不同宿主来源的14株产气荚膜梭菌进行了16S rRNA基因的同源性分析。结果显示,患病鸡肠道分离到的产气荚膜梭菌均为A型且肠道内容物活菌数量达106 CFU/g以上;动物试验结果显示,攻毒菌液上清的小鼠在观察期内死亡并表现出明显的坏死性肠炎症状;药敏试验结果表明,不同来源菌株耐药性存在差异;同源性分析发现,不同宿主来源的14株产气荚膜梭菌无明显宿主特异性。本试验结果可为临床肉鸡梭菌性肠炎的了解及防治提供一定的参考价值。
To investigate the incidence of commercial broiler Clostridium enteritis,Clostridium perfringens were isolated and identified from the intestinal contents of broilers with enteritis symptoms of different feeding patterns,and then a viable count,toxin type identification and animal challenge experiment were carried out.Drug sensitivity test was operated on 43 isolated strains form Clostridium enteritis.Furthermore,homological analysis was carried out on 14 strains of Clostridium perfringens isolated from different hosts by 16S rRNA gene.Results showed that all Clostridium perfringens isolated from intestinal tract of the diseased broilers were identified to be type A and number of viable bacteria in the intestinal contents were all above 106 CFU/g.Results of animal challenge experiment showed that mice challenged with cultural supernatant of the isolated bacterium were all died during the observation period and had obvious symptoms of necrotic enteritis.Results of drug susceptibility test indicated that there were differences in drug resistance of the tested strains.Homological analysis discovered that 14 strains of Clostridium perfringens had no obvious host specificity.The study can provide certain reference value for clinical broiler necrotic enteritis,and then prevention and treatment of the disease.
作者
张寒
孔垂民
刘宗柱
刘文华
ZHANG Han;KONG Chui-min;LIU Zong-zhu;LIU Wen-hua(College of Veterinary Science,Qingdao Agricultural University,Qingdao 266109,China)
出处
《中国兽医杂志》
CAS
北大核心
2020年第6期35-38,I0003,共5页
Chinese Journal of Veterinary Medicine
基金
国家重点研发计划(2018YFD0501400)
山东省重点研发计划项目(2019GNC106058)。
关键词
商品肉鸡
产气荚膜梭菌
药敏试验
同源性分析
commercial broiler
Clostridium perfringens
drug sensitivity test
homology analysis
