摘要
目的探讨套管法在小型猪可逆性单侧输尿管部分梗阻(RPUUO)模型中应用的效果。方法2019年6月至12月,将成年健康雄性贵州小型猪9只(贵州中医药大学动物研究所提供)切除左肾,随机抽签分为3组,分别为假手术组(S组)、持续性单侧输尿管部分梗阻组(CPUUO组)和RPUUO组,每组3只。RPUUO组和CPUUO组用套管法造成右侧输尿管部分梗阻。术后第14天,RPUUO组解除梗阻,CPUUO组不解除梗阻。术前和术后第3、7、14、28天,抽血查肌酐(SCr)和尿素氮(BUN),超声扫描观察肾脏集合系统分离程度(DRCSS)。术后第28天处死全部猪,取右肾行苏木精-伊红(HE)染色,观察肾脏病理损伤,组间比较采用单因素重复测量数据的方差分析。结果RPUUO组SCr和BUN在术后第3天较术前明显升高,第14天达最高值,在第28天恢复到接近术前水平;在术后第7、14天较S组高[SCr:(133.11±14.73)μmol/L比(114.43±4.40)μmol/L、(169.13±13.68)μmol/L比(115.53±5.90)μmol/L,t=19.374、24.267,P<0.05),BUN:(5.81±0.85)mmol/L比(5.81±0.85)mmol/L、(7.96±0.41)mmol/L比(7.96±0.41)mmol/L,t=18.083、30.760,P<0.05];在术后第28天比CPUUO组低[SCr:(111.33±12.02)μmol/L比(268.03±14.84)μmol/L,t=16.683,P<0.05),BUN(5.10±0.15)mmol/L比(15.21±0.54)mmol/L,t=22.408,P<0.05)。RPUUO组DRCSS在术后第7天增加,第14天达最大值,在第28天恢复到接近术前水平;在术后第7、14天较S组增大[(2.23±0.18)mmol/L比(0.61±0.09)mmol/L、(3.90±0.19)mmol/L比(0.60±0.08)mmol/L,t=20.308、28.675,P<0.05];在术后第28天比CPUUO组低[(0.94±0.39)mmol/L比(5.93±0.23)mmol/L,t=16.474,P<0.05]。S组肾脏组织结构未见明显病理改变。CPUUO组肾脏组织结构重度异常,肾小球数量明显减少,肾小管大面积萎缩消失,残存肾小管明显扩张,肾间质内大量成纤维细胞增生及炎性细胞浸润。RPUUO组肾小球变化不明显,肾小管轻度扩张,肾间质未见明显炎性细胞浸润。结论套管法在小型猪上建立RPUUO模型,这种�
Objective To explore the effect of cannula in the RPUUO model of miniature pigs.Methods Nine adult healthy male Guizhou miniature pigs,left kidney of which had been removed,were randomly divided into three groups:S group,CPUUO group and RPUUO group,three in each group.In the RPUUO group and the CPUUO group,right ureteral obstruction was caused by cannula.On the 14th day after operation,the obstruction was relieved in the RPUUO group and not in the CPUUO group.Before and on the 3rd,7th,14th and 28th day after the operation,blood samples were taken to check the SCr and BUN,and DRCSS was observed by ultrasound scanning.On the 28th day after operation,all pigs were sacrificed and the right kidney was stained with HE to observe the renal pathological damage.Statistical analysis using SPSS 21.0.Results The SCr and BUN of the RPUUO group increased significantly on the 3rd day after operation,reached the highest value on the 14th day,and recovered on the 28th day.The SCr and BUN of the RPUUO group was higher than those of the S group on the 7th and 14th day after operation SCr[(133.11±14.73)μmol/L vs.114.43±4.40)μmol/L,(169.13±13.68)μmol/L vs.(115.53±5.90)μmol/L,t=19.374,24.267,P<0.05],BUN[(5.81±0.85)mmol/L vs.(5.81±0.85)mmol/L,(7.96±0.41)mmol/L vs.(7.96±0.41)mmol/L,t=18.083,30.760,P<0.05].On the 28th day,the SCr and BUN of the RPUUO group was lower than those of CPUUO group SCr[(111.33±12.02)μmol/L vs.(268.03±14.84)μmol/L,t=16.683,P<0.05],BUN[(5.10±0.15)mmol/L vs.(15.21±0.54)mmol/L,t=22.408,P<0.05].The DRCSS of the RPUUO group increased significantly on the 7th day after operation,reached the highest value on the 14th day,and recovered on the 28th day.The DRCSS of the RPUUO group was higher than that of the S group on the 7th and 14th day after operation[(2.23±0.18)mmol/L vs.(0.61±0.09)mmol/L,(3.90±0.19)mmol/L vs.(0.60±0.08)mmol/L,t=20.308,28.675,P<0.05].On the 28th day,the DRCSS of the RPUUO group was lower than that of CPUUO group[(0.94±0.39)mmol/L vs.(5.93±0.23)mmol/L,t=16.474,P<0.05].No obvio
作者
宋赏
石华
裴军
文饶
余丹丹
杨超
徐元高
李凯
孙发
徐述雄
Song Shang;Shi Hua;Pei Jun;Wen Rao;Yu Dandan;Yang Chao;Xu Yuangao;Li Kai;Sun Fa;Xu Shuxiong(Graduate School,Zunyi Medical University,Zunyi 563003,China;Department of Urology,Guizhou Provincial People’s Hospital,Guiyang,550002,China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2020年第8期1442-1445,共4页
Chinese Journal of Experimental Surgery
基金
贵州省科技计划项目(黔科合LH[2015]7145)
贵阳市科技计划项目(筑科合同[20161001]31)
贵州省高层次创新型人才培养(GZSYQCC[2014]005)。
关键词
单侧输尿管部分梗阻
动物模型
可逆性
肾积水
Partial unilateral ureteral obstruction
Animal model
Reversible
Hydronephrosis
