摘要
该研究利用16S rRNA扩增子测序技术分析了中国热带(新村、黎安、潭门)典型海草床水体潜在致病菌的群落结构,通过选择性培养基分析可培养弧菌和肠球菌的丰度,进一步利用定量PCR分析弧菌(Vibrio)和肠球菌(Enterococcus)典型毒力基因含量。结果发现,新村、黎安海草床水体潜在致病菌与潭门有显著差异;可培养弧菌和肠球菌及其典型毒力基因vhh、toxR、aspA、esp和gelE均呈现新村>黎安>潭门的趋势,而弧菌典型毒力基因ctxA、tlh、trh和vvp呈现黎安>新村>潭门的趋势。新村和黎安较高的人类活动强度如营养负荷,可能是导致其海草床致病菌和毒力基因丰度较高的重要原因,而新村和黎安的差异可能与不同的人类活动方式有关。
In this study,16S rRNA gene sequencing was used to analyze the community structure of potential pathogenic bacteria in the typical tropical(Xincun,Li'an,Tanmen)seagrass meadows of the South China Sea.The abundances of Vibrio and Enterococcus were counted by selective medium analysis,and their typical virulence genes abundances were quantified by quantitative PCR.The results show that the putative bacterial pathogens in Xincun and Li'an clustered and then separated from those of Tanmen.Abundances of cultured Vibrio and Enterococcus,and the typical virulence genes contents of vhh,toxR,aspA,esp,and gelE followed a descending order of Xincun>Li'an>Tanmen,while the contents of Vibrio typical virulence genes of ctxA,tlh,trh,and vvp followed a descending order of Tanmen>Xincun>Li'an.Higher intensity human activities,such as nutrient load,probably are responsible for the higher abundance of pathogens and virulence genes of seagrass meadows in Xincun and Li'an,while the differences between Xincun and Li'an may be attributed to different types of human activities.
作者
邓益琴
刘松林
冯娟
江志坚
DENG Yiqin;LIU Songlin;FENG Juan;JIANG Zhijian(South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment/Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China;South China Sea Institute of Oceanology,Chinese Academy of Sciences/Key Laboratory of Tropical Marine Bio-resources and Ecology,Guangzhou 510301,China)
出处
《南方水产科学》
CAS
CSCD
北大核心
2020年第5期1-9,共9页
South China Fisheries Science
基金
国家自然科学基金项目(31902415,41806147)
广东省自然科学基金项目(2019A1515011833)
中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2019TS04)
中国水产科学研究院基本科研业务费专项资金(2019ZD0707)
广东省渔业生态环境重点实验室开放基金(FEEL-2017-5)。
关键词
海草床
潜在致病菌
弧菌
肠球菌
典型毒力基因
Seagrass meadow
Potential pathogens
Vibrio
Enterococcus
Typical virulence gene
