摘要
为促进布鲁菌病Rev.1疫苗及相关疫苗的研发,该研究提取Rev.1疫苗株核酸,应用PacBio平台进行全基因序列测定与分析。结果表明,Rev.1疫苗株基因组大小约3299187 bp,G+C含量为57.2%,组装为染色体1、染色体2两条环状基因组,大小分别为2121370、1177817 bp,G+C含量分别为57.2%、57.3%。将其Ery、BLS和VirB10基因序列与9株GenBank上发表的布鲁菌参考菌株的Ery、BLS和VirB10基因序列进行比较分析,存在不同程度的差异,同源性为97.4%~100%。
In order to promote the research and development of Brucella Rev.1 vaccine and related vaccines,the nucleic acid of the Brucella Rev.1 vaccine strain was extracted,and the genome-wide sequencing for Brucella Rev.1 vaccine strain was carried out and analyzed through PacBio platform.The results showed that the full-length genome of the Brucella Rev.1 vaccine strain was 3299187 bp,and the G+C content was 57.2%;two circular genomes of chromosome 1 and chromosome 2 were assembled;the size of chromosome 1 and chromosome 2 was 2121370 bp and 1177817 bp,respectively,and the G+C content of chromosome 1 and chromosome 2 was 57.2%and 57.3%,respectively.The sequences of Ery,BLS and VirB10 genes of the Brucella Rev.1 vaccine strain were bioinformatically compared and analyzed with their counterparts of 9 Brucella reference strains,and the sequence similarity among those genes were observed,ranging from 97.4%to 100%.
作者
于环宇
张志丹
刘建奇
赵丹彤
赵丽霞
刘国英
宋庆庆
王国华
潘相臣
刘冬冬
田普厚
石顺利
关平原
YU Huan-yu;ZHANG Zhi-dan;LIU Jian-qi;ZHAO Dan-tong;ZHAO Li-xia;LIU Guo-ying;SONG Qing-qing;WANG Guo-hua;PAN Xiang-chen;LIU Dong-dong;TIAN Pu-hou;SHI Shun-li;GUAN Ping-yuan(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot010018,China;National Engineering Laboratory for Veterinary Vaccine,The Spirit Jinyu Biological Pharmaceutical Co.,Ltd.,Hohhot010030,China;Inner Mongolia Bigvet Biotech Co.,Ltd.,Hohhot011500,China;Livestock Breeding Guide Station of Tongliao City of Inner Mongolia,Tongliao028000,China)
出处
《畜牧与饲料科学》
2020年第5期91-95,共5页
Animal Husbandry and Feed Science
基金
内蒙古自治区科技计划项目“布鲁菌病免疫方法及新型可鉴别疫苗的开发”(201702165)。
