摘要
目的:评价右美托咪定对小鼠大脑初级体感皮层快放电抑制性中间神经元兴奋性的影响。方法:出生15~21 d健康C57BL/6小鼠11只,雌雄不拘,制备含有初级体感皮层的新鲜脑片,采用人工脑脊液孵育,应用膜片钳技术分别在第四层快放电中间神经元和兴奋性神经元上建立全细胞记录模式。于加入右美托咪定(10μmol/L)前和加入后5 min时记录快放电抑制性中间神经元的膜电位和动作电位阈刺激强度及兴奋性神经元自发抑制性突触后电流。结果:与加入右美托咪定前比较,加入右美托咪定后5 min时大脑初级体感皮层快放电抑制性中间神经元膜电位、阈刺激强度和兴奋性神经元自发抑制性突触后电流的频率和波幅差异均无统计学意义(P>0.05)。结论:右美托咪定镇痛的机制可能与大脑初级体感皮层快放电抑制性中间神经元的兴奋性无关。
Objective To evaluate the effect of dexmedetomidine on the excitability of fast-spiking interneurons in the primary somatosensory cortex of mouse brain.Methods Eleven healthy C57BL/6 mice of either sex,at postnatal days 15-21,were used.The acute brain slices of primary somatosensory cortex were prepared and incubated in artificial cerebrospinal fluid.The whole-cell patch-clamp was established on fast-spiking interneurons and excitatory neurons in primary somatosensory cortex layer 4 separately.The membrane potential and action potential threshold from fast-spiking interneurons and spontaneous inhibitory postsynaptic currents from excitatory neurons were recorded before application of dexmedetomidine(10μmol/L)and at 5 min after application of dexmedetomidine.Results Compared with that before application of dexmedetomidine,no significant change was found in the membrane potential and action potential threshold of fast-spiking interneurons and frequency and amplitude of spontaneous inhibitory postsynaptic currents at 5 min after application of dexmedetomidine(P>0.05).Conclusion The analgesic mechanism of dexmedetomidine may not be related to the excitability of fast-spiking interneurons in the primary somatosensory cortex of the mouse brain.
作者
唐佳丽
汤博
黄宇光
Tang Jiali;Tang Bo;Huang Yuguang(Department of Anesthesiology,Peking Union Medical College Hospital Chinese Academy of Medical Sciences,Beijing 100730,China)
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2020年第6期698-702,共5页
Chinese Journal of Anesthesiology
基金
北京市自然科学基金(7184233)。
