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一测多评法同时测定补肾防喘片中7种成分的含量 被引量:2

Quality control for 7 components in Bushen Fangchuan Tablets by QAMS
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摘要 目的建立一测多评法(QAMS)同时测定补肾防喘片中7种成分的含量,并验证该方法的准确性及可行性。方法选取Agilent SB-C 18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-2 mL·L^-1磷酸,梯度洗脱;流速为1.0 mL·min^-1;柱温为30℃。以补骨脂素为内参物,建立其他6种成分的相对校正因子,计算各成分含量。结果毛蕊花糖苷、吉奥诺苷B 1、马替诺皂苷、补骨脂素、异补骨脂素、淫羊藿苷和宝藿苷Ⅰ的质量浓度分别在3.09~61.80,1.17~23.40,1.39~27.80,4.91~98.20,4.18~83.60,5.07~101.40和2.36~47.20μg·mL^-1范围内线性关系良好(r≥0.9991);平均回收率(RSD值)分别为99.36%(0.93%),98.32%(1.38%),96.90%(1.06%),98.93%(1.11%),100.01%(0.70%),99.50%(0.89%)和97.93%(1.21%);QAMS法计算结果与外标法(ESM)实测值无明显差异。结论该方法操作简便,结果准确,可同时测定补肾防喘片中7种成分的含量。 Objective To establish a quantitative analysis of multi-components by single marker(QAMS)method for the determination of 7 components in Bushen Fangchuan Tablets,and to verify the accuracy and feasibility of the method.Methods An HPLC method with Agilent SB-C 18(250 mm×4.6 mm,5μm)column was applied,the mobile phase was acetonitrile-2 mL·L^-1 phosphoric acid,gradient elution,the flow rate was 1.0 mL·min^-1,and the column temperature was 30℃.By using psoralen as an internal standard,the relative correction factors of the other 6 constituents were calculated,after which the content determination was made.Results Verbascoside,jionoside B 1,martynoside,psoralen,isopsoralen,icariin and baohuosideⅠshowed good linear relationships within the ranges of 3.09-61.80,1.17-23.40,1.39-27.80,4.91-98.20,4.18-83.60,5.07-101.40 and 2.36-47.20μg·mL^-1(r≥0.9991),whose average recoveries(RSDs)were 99.36%(0.93%),98.32%(1.38%),96.90%(1.06%),98.93%(1.11%),100.01%(0.70%),99.50%(0.89%)and 97.93%(1.21%),respectively.The results obtained by QAMS approximated to those obtained by external standard method(ESM).Conclusion The method is simple and accurate,and can be used to simultaneously determine the content of 7 components in Bushen Fangchuan Tablets.
作者 黄永辉 杨元飞 陈勇 HUANG Yonghui;YANG Yuanfei;CHEN Yong(Department of Pharmacy,People′s Hospital of Dazhu County,Dazhu 635100,China;Chengdu Diao Group Tianfu Pharmaceutical Limited Company,Dazhou 635027,China)
出处 《西北药学杂志》 CAS 2020年第5期653-658,共6页 Northwest Pharmaceutical Journal
关键词 一测多评法 补肾防喘片 相对校正因子 含量测定 quantitative analysis of multi-components by single-marker(QAMS) Bushen Fangchuan Tablets relative correction factor content determination
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