摘要
目的鉴定一种用于抗血清补体干扰的绿色环保型免疫检测阻断剂。方法用抗体抗原反应缓冲液将聚天冬氨酸(钠)配制成系列浓度;再用聚天冬氨酸钠溶液处理临床血清样本;接着用制备的癌胚抗原(CEA)检测试剂检测处理后的血清样本;最后与商业化罗氏试剂对比检测结果,并评价聚天冬氨酸钠抗血清补体干扰的效果。结果临床血清样本经聚天冬氨酸(钠)溶液处理后,可以提高免疫检测试剂抗血清补体干扰作用。与罗氏试剂对比,阻断剂添加前后相关性R2值从0.937 5提高到0.954 5。优于EDTA的处理效果(r2值为0.943 0)。结论聚天冬氨酸(钠)作为绿色环保型除垢剂,可以用于免疫检测试剂中。聚天冬氨酸(钠)的加入有利于增强试剂抗血清干扰能力,提高检测结果的准确性。
Objective To identify a green environmental friendly immunoassay blocker against serum complement interference. Methods A series of concentrations of polyaspartic acid(sodium) were prepared with antibody and antigen reaction buffer. The serum samples were treated with polyaspartic acid sodium solution,and then the pretreated serum samples were detected with the prepared CEA reagent. Finally,the results were compared with commercial Roche reagent,and the effect of anti-interference of polyaspartic acid sodium was evaluated. Results After clinical serum samples are treated with polyaspartic acid(sodium)solution,the anti-serum complement interference effect of immunoassay reagents can be improved. Compared with Roches reagent,the correlation R2 value before and after the addition of the blocking agent increased from0.937 5 to 0.954 5. The treatment effect is better than EDTA(R2 value is 0.943 0). Conclusion Polyaspartic acid(sodium),as a green environmentally friendly detergent,can be used in immunoassay reagents. The addition of polyaspartic acid(sodium)is beneficial to enhance the reagents anti-serum interference ability and improve the accuracy of detection results.
作者
陈立勇
刘仁源
张瑜
CHEN Liyong;LIU Renyuan;ZHANG Yu(Dongguan Dongyangguang Diagnostic Products Co.,Ltd,Dongguan,Guangdong,China,523867)
出处
《分子诊断与治疗杂志》
2020年第9期1200-1203,1212,共5页
Journal of Molecular Diagnostics and Therapy
