摘要
目的研究黄精对非酒精性脂肪肝(NAFLD)大鼠的保肝作用及对氧化应激的调控作用。方法SD大鼠饲喂甲硫氨酸胆碱缺乏(MCD)饲料进行NAFLD模型的建立,同时选取10只大鼠为对照组饲喂正常饲料,将构建成功的NAFLD大鼠随机分为模型组和低、高剂量实验组,每组15只。低、高剂量实验组分别灌胃黄精水提物(生药量3,6 mg·kg^-1),对照组和模型组灌胃等量生理盐水,每天1次,连续灌服3个月。以全自动生化分析仪检测血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)水平;以油红染色法观察大鼠肝组织病理变化;以蛋白质印迹法检测大鼠肝组织中CYP2E1蛋白表达量,以试剂盒检测大鼠肝组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、髓过氧化物酶(MPO)水平。结果干预后,对照组、模型组和低、高剂量实验组大鼠血清GPT水平分别为(43.15±4.15),(93.15±5.86),(82.16±6.33),(56.15±4.72)U·L^-1,GOT水平分别为(96.48±14.75),(192.45±13.25),(172.16±10.42),(133.26±4.68)U·L^-1,肝组织中MDA水平分别为(5.89±1.24),(10.22±2.15),(8.69±2.41),(6.58±1.48)nmol·mg^-1,SOD水平分别为(196.42±12.85),(168.45±6.48),(174.65±10.85),(188.63±12.05)U·mg^-1,MPO水平分别为(128.64±25.68),(289.46±24.46),(243.46±17.55),(185.26±26.71)kU·g^-1,GSH水平分别为(73.56±15.26),(31.45±11.53),(68.33±9.03),(45.26±10.42)mg·g^-1,肝组织CYP2E1蛋白相对表达量分别为0.11±0.04,0.91±0.10,0.32±0.04,0.10±0.03,差异均有统计学意义(均P<0.05)。结论黄精水提物可调控NAFLD模型大鼠血清氧化应激因子及肝组织CYP2E1蛋白表达水平进而起到改善肝组织形态及肝功能的效果。
Objective To investigate the hepatoprotective effect of Rhizoma Polygonatum on non-alcoholic fatty liver(NAFLD)rats and its regulatory effect on oxidative stress.Methods The NAFLD model was established by administering SD rats with methionine choline deficiency(MCD)feed.At the same time,10 rats were selected as control group and fed with normal feed.The successful modeled NAFLD rats were randomly divided into model group and Exp-L/H groups,15 rats in each group.Exp-L/H groups were administered with Rhizoma Polygonatum water extract(raw drug 3,6 mg·kg^-1)by gavage,control group and model group were administered with the same amount of normal saline by gavage,once a day,and continuously for 3 months.Fully automatic biochemical analyzer was used to detect serum glutamic-pyruvic transaminase(GPT)and glutamic-oxaloacetic transaminase(GOT)levels;oil red staining was used to observe pathological changes in rat liver tissue;Western blot was used to detect CYP2 E1 protein expression in rat liver tissue,the levels of malondialdehyde(MDA),superoxide dismutase(SOD),glutathione(GSH)and myeloperoxidase(MPO)in rat liver tissues were detected by kit.Results After intervention,the serum GPT levels of control group,model group and Exp-L/H groups were(43.15±4.15),(93.15±5.86),(82.16±6.33),(56.15±4.72)U·L^-1,GOT levels were(96.48±14.75),(192.45±13.25),(172.16±10.42),(133.26±4.68)U·L^-1,the MDA in the liver tissues were(5.89±1.24),(10.22±2.15),(8.69±2.41),(6.58±1.48)nmol·mg^-1,SOD were(196.42±12.85),(168.45±6.48),(174.65±10.85),(188.63±12.05)U·mg^-1,MPO were(128.64±25.68),(289.46±24.46),(243.46±17.55),(185.26±26.71)kU·g^-1,the GSH were(73.56±15.26),(31.45±11.53),(68.33±9.03),(45.26±10.42)mg·g^-1,the relative expression of CYP2 E1 protein in liver tissues were 0.11±0.04,0.91±0.10,0.32±0.04,0.10±0.03,the differences were all with statistically significant(all P<0.05).Conclusion The water extract of Rhizoma Polygonatum can regulate the expression of oxidative stress factor and CYP2 E1 protein in liver
作者
王建忠
兰少波
黄敏敏
WANG Jian-zhong;LAN Shao-bo;HUANG Min-min(Department of Infectious Diseases,Affiliated Hospital of Shaoxing University,Shaoxing 312000,Zhejiang Province,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2020年第17期2650-2653,共4页
The Chinese Journal of Clinical Pharmacology
关键词
非酒精性脂肪性肝病
黄精
氧化应激
肝功能
non-alcoholic fatty liver disease
Rhizoma Polygonatum
oxidative stress
liver function
