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miR-135b-5p靶向CMTM3调节胃癌细胞的增殖、侵袭和迁移能力 被引量:3

miR-135b-5p targets CMTM3 to regulate the proliferation,invasion and migration of gastric cancer cells
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摘要 目的:探究miR-135b-5p通过靶向CMTM3基因调节胃癌细胞的增殖、侵袭和迁移能力。方法:采用实时荧光定量PCR(RT-qPCR)检测胃癌组织和细胞系中CMTM3和miR-135b-5p的表达水平。利用慢性病毒转染技术将miR-135b-5p inhibitor转染至胃癌细胞,RT-qPCR检测转染效率;CCK-8比色法和Transwell实验检测转染后细胞的增殖、侵袭和迁移能力;Western blotting检测转染后细胞中CMTM3蛋白表达水平。利用生物信息学网站预测miR-135b-5p潜在靶基因CMTM3,利用荧光素酶实验进行验证。结果:RT-qPCR检测结果表明,miR-135b-5p在胃癌组织和胃癌细胞株中呈现高表达,CMTM3在胃癌组织中呈现低表达(P<0.01)。RT-qPCR检测转染效率,结果显示,转染miR-135b-5p inhibitor敲低了miR-135b-5p的表达水平(P<0.001);CCK-8和Transwell实验结果表明,敲低miR-135b-5p后抑制了胃癌细胞的增殖、侵袭和迁移能力(P<0.01),Western blotting实验结果表明,敲低miR-135b-5p促进了CMTM3蛋白的表达。生物信息学网站预测CMTM3为miR-135b-5p的潜在靶基因,荧光素酶实验证实了miR-135b-5p直接靶向胃癌细胞中CMTM3基因(P<0.01)。结论:miR-135b-5p作为重要的调节因子,反向调节抑癌靶基因CMTM3,从而促进胃癌细胞的增殖、侵袭和迁移能力。 Objective:To explore the ability of miR-135 b-5 p to regulate the proliferation,invasion and migration of gastric cancer cells by targeting the CMTM3 gene.Methods:The expression of CMTM3 and miR-135 b-5 p in gastric cancer tissues and cells was detected by RT-qPCR.miR-135 b-5 p inhibitor was transferred into gastric cancer cells by chronic virus transfection technique,and the transfection efficiency was detected by RT-qPCR.CCK-8 colorimetric assay and Transwell test were used to detect the proliferation,invasion and migration ability of gastric cancer cells transfected with miR-135 b-5 p inhibitor,respectively.The expression level of CMTM3 protein after transfection was detected by Western blotting.The potential target gene CMTM3 of miR-135 b-5 p was predicted by the biological information website,and the luciferase experiment was used to verify the results.Results:The results of RT-qPCR showed that miR-135 b-5 p was highly expressed in gastric cancer tissue and gastric cancer cells,and CMTM3 showed low expression in gastric cancer tissue(P<0.01).The results of RT-qPCR detection of transfection efficiency showed that the expression level of miR-135 b-5 p was decreased by transfection of miR-135 b-5 p inhibitor(P<0.001).The results of vitro transfection experiments showed that the proliferation,invasion and migration ability of gastric cancer cells were inhibited by knockdown of miR-135 b-5 p(P<0.01).The results of Western blotting assay showed that knockdown of miR-135 b-5 p promoted the expression of CMTM3 protein.The bioinformatics website predicted that CMTM3 was a potential target gene of miR-135 b-5 p,and the fluciferase test confirmed that miR-135 b-5 p was directly targeted at CMTM3 gene(P<0.01).Conclusion:As an important regulatory factor,miR-135 b-5 p inversely regulates the target gene CMTM3,which promotes the proliferation,invasion and migration of gastric cancer cells.
作者 李永红 刘文明 李伟 董链 杨峥嵘 衷敬华 Li Yonghong;Liu Wenming;Li Wei;Dong Lian;Yang Zhengrong;Zhong Jinghua(Oncology Department,Tianmen First People's Hospital,Hubei Tianmen 431700,China;Hubei Key Laboratory of Occupational Hazard Identification and Control,Wuhan University of Science and Technology,Hubei Wuhan 430081,China;Oncology Department,the First Affiliated Hospital of Gannan Medical University,Jiangxi Ganzhou 341000,China)
出处 《现代肿瘤医学》 CAS 2020年第19期3305-3310,共6页 Journal of Modern Oncology
基金 湖北省卫生健康科研基金资助(编号:WJ2019H211)。
关键词 miR-135b-5p 胃癌 CMTM3 miR-135b-5p gastric cancer CMTM3
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  • 1Zhu, Yi-Min,Zhong, Zheng-Xiang,Liu, Zhi-Ming.Relationship between let-7a and gastric mucosa cancerization and its significance[J].World Journal of Gastroenterology,2010,16(26):3325-3329. 被引量:17
  • 2Bartel DP. MicroRNAs : target recognition and regulatory functions. Cell, 2009, 136: 215-233. 被引量:1
  • 3Lewis BP, Burge CB, Bartel DP. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell, 2005, 120 : 15-20. 被引量:1
  • 4Duan R, Pak C, Jin P. Single nucleotide polymorphism associated with mature miR-125a alters the processing of pri-miRNA. Hum Mol Genet, 2007, 16:1124-1131. 被引量:1
  • 5Lulla RR, Costa FF, Bischof JM, et al. Identification of differentially expressed microRNAs in Osteosarcoma. Sarcoma, 2011:732690. 被引量:1
  • 6Sarver AL, French A J, Borralho PM, et al. Human colon cancer profiles show differential microRNA expression depending on mismatch repair status and are characteristic of undifferentiated proliferative states. BMC Cancer, 2009, 9:401. 被引量:1
  • 7Tong AW, Fulgham P, Jay C, et al. MicroRNA profile analysis of human prostate cancers. Cancer Gene Ther, 2009, 16:206-216. 被引量:1
  • 8Yu F, Yao H, Zhu P, et al. let-7 regulates self renewal and tumorigenicity of breast cancer cells. Cell, 2007, 131 : 1109-1123. 被引量:1
  • 9Steinemann D, Tauscher M, Praulich I, et al. Mutations in the let-7 binding site-a mechanism of RAS activation in juvenile myelomonocytic leukemia?. Haematologica, 2010, 95:1616. 被引量:1
  • 10Wong TS, Man OY, Tsang CM, et al. MicroRNA let-7 suppresses nasopharyngeal carcinoma ceils proliferation through downregulating c-Myc expression. J Cancer Res Clin Oncol, 2011,137: 415-422. 被引量:1

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