摘要
本文以甘肃兰州百合为原料,按照DB62/T 412标准依次进行分级,选取百合(百合片宽度≤14.90 mm)的芯为原料,探究了热水浸提法提取百合芯多糖的工艺条件,并对多糖的抗氧化活性进行了比较分析。在单因素试验的基础上,选取料液比、提取时间和提取温度进行三因素三水平Box-Benhnken试验,利用Design-Expert 11.0软件进行试验数据分析,得到最佳提取条件,并对最佳提取条件的粗多糖进行抗氧化活性的研究。结果表明,百合芯多糖的最佳提取条件是料液比为1:11.50,提取温度为69.18℃,提取时间为53.82 min,在此条件下多糖得率为10.30%,与响应面试验验证结果为10.29%。相对误差0.09%,误差较小。百合芯多糖在不同的抗氧化体系中有不同的抗氧化活性,百合芯多糖浓度在1.0 mg/mL时抗氧化活性最佳,对·OH清除率为65.00%,对DPPH·清除率为58.00%,但百合芯多糖的还原力较弱且远远低于同浓度的Vc还原力。因此,Box-Benhnke响应面法可用于百合芯多糖提取工艺,优化效果好,同时百合芯多糖有一定的抗氧化活性,百合芯可以在多糖提取方面应用,以便于百合资源的最大化利用。
The Lanzhou lily core in Gansu Province,China was used as the raw material for this study.According to the DB62/T 412 standard for grading,the core(lily slice width≤14.90 mm)was extracted from the lily.The technological conditions for isolating polysaccharides from lily cores by hot water extraction were studied,and the antioxidant activity was compared and analyzed.Based on the single-factor test,the material-liquid ratio,extraction time,and extraction temperature were selected for the three-factor three-level Box-Benhnken test.The experiment data were analyzed using Design-Expert 11.0 software to obtain the best extraction conditions,and the crude polysaccharides that were extracted under the best conditions were evaluated for antioxidant activity.The results showed that the best lily core polysaccharide extraction conditions were a material-liquid ratio of 1:11.50,an extraction temperature of 69.18℃,and an extraction time of 53.82 min.Under these conditions,the polysaccharide yield was 10.30%,and the response surface test verification result was 10.29%.The relative error was 0.09%,which was relatively small.Lily core polysaccharides had different antioxidant activities in different antioxidant systems.Specifically,lily core polysaccharides had the best antioxidant activity at a concentration of 1.0 mg/mL,with an OH scavenging rate of 65.00%and a DPPH scavenging rate of 58.00%.However,the reducing power of lily core polysaccharides was weak and much lower than that of Vc at the same concentration.Therefore,the Box-Benhnken response surface method can be used in the lily core polysaccharide extraction process,and the optimization effect is good.At the same time,lily core polysaccharides have certain antioxidant activities,and the extraction of polysaccharides can be applied to lily cores in order to maximize the utilization of lily resources.
作者
王昭珺
刘晓风
徐也
WANG Zhao-jun;LIU Xiao-feng;XU Ye(School of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,China)
出处
《现代食品科技》
EI
CAS
北大核心
2020年第9期219-227,201,共10页
Modern Food Science and Technology
基金
国家自然科学基金资助项目(81660581)。
关键词
兰州百合
多糖提取
响应面优化
抗氧化
热水浸提
Lanzhou lily
polysaccharide extraction
response surface optimization
antioxidant
hot water extraction
