摘要
目的研究和建立一种基于96孔板-酶标仪的水中氟离子含量的高通量快速检测方法(简称本方法),并对其进行性能评价。方法根据氟离子与氟试剂及硝酸镧反应生成蓝色三元络合物的原理,络合物在650 nm波长处的吸光度值与氟离子浓度成正比,利用酶标仪定量测定水样中的氟离子含量。通过检测加标回收率、标准物质等方法评价本方法的准确度、精确度和检测限。结果本方法标准曲线线性相关系数>0.999;准确度实验中不同氟浓度的加标回收率在99.00%~103.33%,平均回收率为101.08%;标准物质法中,测定值均在标准溶液的标定值范围内;精密度实验中不同氟浓度水样的相对标准偏差均≤10%;检测限为0.06 mg/L。结论本方法检测结果的准确度、精密度均较好,检测限低,样本用量少,可极大提高检测工作效率。
Objective To study and establish a high-throughput and rapid detection method for fluorine ion content in water based on 96-well plate and microplate reader,and to evaluate its performance.Methods According to the principle of blue ternary complex formed by reaction of fluorine ion with fluorine reagent and lanthanum nitrate,the absorbance of the complex at the wavelength of 650 nm was proportional to the concentration of fluoride ion,and the content of fluoride ion in water samples was quantitatively determined by microplate reader.The accuracy,precision and detection limit of the method were evaluated by the standard recovery rate method and the reference material method.Results The linear correlation coefficient of the standard curve of this method was>0.999;the standard recovery rates of different concentrations were 99.00%-103.33%in the accuracy experiment of this method,and the average recovery rate was 101.08%.In the standard material method,the measured values are all within the calibration range of the standard solution.The relative standard deviation of water samples with different concentrations was≤10%in the precision experiment and the detection limit was 0.06 mg/L.Conclusion The method has good accuracy and precision,low detection limit and small sample consumption,and this method can greatly improve the detection efficiency.
作者
武辽伟
霍思梦
褚衍茹
黄伟
王健
杨艳梅
高彦辉
王伟
Wu Liaowei;Huo Simeng;Chu Yanru;Huang Wei;Wang Jian;Yang Yanmei;Gao Yanhui;Wang Wei(Institute for Endemic Fluorosis Control,Center for Endemic Disease Control,Chinese Center for Disease Control and Prevention,Key Lab of Etiology and Epidemiology of National Health Commission,Harbin Medical University,Harbin 150081,China)
出处
《中华地方病学杂志》
CAS
CSCD
北大核心
2020年第9期692-696,共5页
Chinese Journal of Endemiology
基金
黑龙江省卫生计生委科研课题(2016-186)
黑龙江省省属高等学校基本科研业务费科研项目(2017JCZX12)
黑龙江省应用技术研究与开发计划重大项目(GY2018ZB0065)。
关键词
氟离子
检测方法
酶标仪
高通量快速
Fluoride ion
Detection method
Microplate reader
High-throughput and rapid
