摘要
目的探讨羊水细胞载玻片原位培养法在产前诊断中的应用价值。方法2716例羊水细胞采用载玻片原位培养法培养,培养成功后制片,用GSL-120全自动核型扫描仪进行进行扫描拍摄和分析。结果2716例羊水细胞经载玻片原位培养后制片、核型分析,成功率达100%;原代培养成功率达98.42%,传代培养率1.58%。共检出异常224例、占8.25%,其中125例21三体、31例18三体、3例13三体、4例45,X、17例47,XXY、5例47,XYY、1例48,XXY,+18、1例48,XXYY、26例结构异常,并鉴别出11例染色体数目异常真嵌合。结论羊水细胞原位培养法结果稳定,成功率高;能够鉴别真假嵌合,提高产前诊断的精准性。
Objective To explore the value of in situ amniocyte culture for prenatal diagnosis.Methods 2716 amniotic fluid samples were cultured in situ on slides.After the culture,the slides were stained,photographed and analyzed.Results All samples were successfully analyzed,with the success rates for primary culture and subculture being 98.42%and 1.58%,respectively.224 samples(8.25%)were detected with chromosomal aberrations,which included 125 cases with trisomy 21,31 with trisomy 18,3 with trisomy 13,4 with 45,X,17 with 47,XXY,5 with 47,XYY,1 with 48,XXY,+18,1 with 48,XXYY,26 with structural chromosomal aberrations,and 11 with mosaicisms for aneuploidies.Conclusion In situ amniocyte culture is stable and has a high success rate,and is capable of identifying true and false mosaicisms,which can improve the accuracy of prenatal diagnosis.
作者
黄道奇
涂华玉
王朝红
王亚群
童克婷
朱健生
Huang Daoqi;Tu Huayu;Wang Chaohong;Wang Yaqun;Tong Keting;Zhu Jiansheng(Center for Genetic Medicine,Anhui Maternal and Child Health Care Hospital Affiliated to Anhui Medical University,Hefei,Anhui 230011,China)
出处
《中华医学遗传学杂志》
CAS
CSCD
2020年第10期1117-1119,共3页
Chinese Journal of Medical Genetics
关键词
羊水
载玻片
原位培养
产前诊断
嵌合体
Amniocyte
Slide
In situ culture
Prenatal diagnosis
Mosaicism