摘要
目的:研究藏药镰荚棘豆Oxytropis falcata Bunge中的黄酮类成分。方法:采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱及制备型高效液相色谱等进行分离纯化,通过超导核磁共振波谱和X-ray单晶衍射等现代谱学技术分析鉴定了化合物的结构。结果:从镰荚棘豆乙醇提取物的乙酸乙酯萃取部位中分离得到16个黄酮类化合物,分别鉴定为:5-羟基-7-甲氧基二氢黄酮(1)、7-羟基二氢黄酮(2)、5,7-二羟基二氢黄酮(3)、7-羟基-4′-甲氧基异黄酮(4)、7-羟基-3′,4′-亚甲二氧基异黄酮(5)、7,5′-二羟基-3′-甲氧基异黄酮(6)、5,7,2′-三羟基-4′-甲氧基异黄酮(7)、苜蓿内酯(8)、5,2′,4′-三羟基-7-甲氧基异黄酮(9)、7,3′-二羟基-4′-甲氧基异黄酮(10)、5,7,2′,4′-四羟基异黄酮(11)、7,5′-二羟基-2′-甲氧基异黄酮(12)、2′-甲氧基-4′-羟基查尔酮(13)、2′,4′-二羟基查尔酮(14)、4,4′-二羟基-6′-甲氧基查尔酮(15)、4,4′-二羟基-2′-甲氧基查尔酮(16)。结论:其中,化合物9、11~13、15、16为首次从该植物中分离得到。
Objective:To investigate the chemical constituents of flavonoids from Tibe medicine Oxytropis falcata.Methods:The silica gel,Sephadex LH-20 column chromatography and preparative high performance liquid chromatography were used to isolate and purify the chemical constituents from Oxytropis falcata.The structures of the isolated compounds were identified by nuclear magnetic resonance spectroscopy and X-ray single crystal diffraction.Results:Sixteen compounds were isolated from the ethyl acetate extract part of Oxytropis falcata ethanol extract,and identified as 5-hydroxy-7-methoxydihydroflavone(1),7-hydroxy dihydroflavone(2),5,7-dihydroxy dihydroflavone(3),7-hydroxy-4′-methoxyisoflavone(4),7-hydroxy-3′,4′-methylene dioxy isoflavone(5),7,5′-dihydroxy-3′-methoxyisoflavone(6),5,7,2′-trihydroxy-4′-methoxyisoflavone(7),medicagol(8),5,2′,4′-trihydroxy-7-methoxyisoflavone(9),7,3′-dihydroxy-4′-methoxyisoflavone(10),5,7,2′,4′-tetrahydroxy isoflavone(11),7,5′-dihydroxy-2′-methoxyisoflavone(12),2′-methoxy-4′-hydroxychalcone(13),2′,4′-dihydroxychalcone(14)4,4′-dihydroxy-6′-methoxychalcone(15),4,4′-dihydroxy-2′-methoxychalcone(16),respectively.Conclusion:Compounds 9,11 to 13,15,16 are isolated from this plant for the first time.
作者
张美荣
杨军丽
姜侃
师彦平
ZHANG Mei-rong;YAHG Jun-li;JIANG Kan;SHI Yan-ping(Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences/CAS Key Laboratory of Chemistry of Northwestern Plant Resources/Key Laboratory of Natural Medicine of Gansu Province,Lanzhou 730000,China;University of Chinese Academy of Sciences,Beijing 100039,China)
出处
《中药材》
CAS
北大核心
2020年第1期71-75,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金项目(21575150,21775153)
中国科学院国际合作局对外合作重点项目资助(153631KYSB20160004)。
关键词
镰荚棘豆
化学成分
二氢黄酮
异黄酮
查尔酮
Oxytropis falcata Bunge
Chemical constituents
Flavonone
Isoflavone
Chalcone
