摘要
[目的]利用生物信息学方法对miRNA-146a在甲状腺癌细胞BCPAP中的作用进行转录组测序,从分子水平研究miRNA-146a对甲状腺癌发病的作用机制,寻找潜在的治疗靶点。[方法]对甲状腺癌细胞BCPAP分别转录miRNA-146a的模拟物、空白模拟物和miRNA-146a的抑制物及空白抑制物,荧光定量PCR检测细胞miRNA-146a的表达;高通量测序检测差异miRNA;对检测结果的差异基因与miRTarBase、miRWalk2.0和TargetScan中miR-146a靶基因数据库共分析,预测miRNA-146a靶基因;用Cluster Profiles 3.0.5软件进行富集分析。[结果]转录组测序结果与miRTarBase、miRWalk2.0和TargetScan中miRNA-146a靶基因数据库共分析,提示miRNA-146a靶基因与CXCL8、CXCR4、IRAK1、PRKCE和LFNG等9个靶基因相关。GO功能注释预测靶基因集合富集在炎症反应、细胞运动及迁移的正调节、趋化性调节、G蛋白偶联受体结合、细胞因子活性及受体结合。KEGG信号通路富集结果提示与Toll样受体信号通路、Notch信号通路、Fc epsilon RI信号通路和趋化因子信号通路等相关。[结论] miRNA-146a在甲状腺癌组织中的表达可能是其分子诊断的有用生物标志物和潜在的靶点,可为甲状腺癌的临床诊断提供一定参考。
[Objective] To study the role of micro RNA-146 a(miRNA-146 a) in thyroid cancer cells and its mechanism with bioinformatics. [Methods] Mi RNA-146 a mimic,blank mimic,inhibitor and blank inhibitor were transcripted in thyroid cancer BCPAP cells,respectively. The expression of miRNA-146 a in thyroid cancer cells was detected by fluorescence quantitative RT-PCR(fq RTPCR). The results were analyzed with miRTar Base,miRWalk2.0 and Target Scan database,and the target gene of miRNA-146 a was predicted. Enrichment analysis was used by Cluster Profiles 3.0.5 software. [Results] The results of transcriptome sequencing were analyzed in the target gene databases of miRNATar Base,micro Walk 2.0 and Target Scan,suggesting that the target genes of miRNA-146 a were related to 9 genes including CXCL8,CXCR4,IRAK1,PRKCE and LFNG.GO functional annotation predicted the accumulation of target genes in inflammatory response,positive regulation of cell movement and migration,chemotaxis regulation,G protein coupled receptor binding,cytokine activity and receptor binding. KEGG signaling pathway enrichment results suggested that Toll-like receptor signaling pathway,Notch signaling pathway,Fc epsilon RI signaling pathway and chemokine signaling pathway were related. [Conclusion] The expression of miRNA-146 a in thyroid cancer tissue may be a useful biomarker and potential target for molecular diagnosis,which may provide some reference for clinical diagnosis of thyroid cancer.
作者
李芳
姜霞
苏兴凯
刘擘
胡毅平
陈英
田延锋
LI Fang;JIANG Xia;SU Xing-kai;LIU Bo;HU Yi-ping;CHEN Ying;TIAN Yan-feng(The First Hospital of Hebei Medical University,Shijiazhuang 050031,China)
出处
《肿瘤学杂志》
CAS
2020年第8期689-694,共6页
Journal of Chinese Oncology
基金
河北省科技计划项目(17277715D)。
