摘要
褐飞虱是造成中国水稻减产最严重的虫害之一,发掘褐飞虱抗性基因是防治水稻褐飞虱的有效途径。籼稻‘ARC5833’是一份高抗褐飞虱的种质材料,为了明确籼稻‘ARC5833’对褐飞虱的抗性机理,以便在育种中加以利用。本研究利用‘ARC5833’与感虫材料‘9311’杂交构建包含145个F2:3家系的作图群体,采用苗期集团法进行褐飞虱抗性鉴定。结果显示,该群体中抗、感家系数分别为109和36,卡方分析表明有1对主效显性基因控制抗虫表型。进一步的基因定位表明,该抗性基因位于水稻第4染色体长臂上的分子标记4M11491与4M24.687之间,遗传距离为4.2 cM。该区间内最大LOD值为40,可以解析表型变异的72%。因该区间内已克隆一个抗褐飞虱基因Bph6,测序分析表明,它们的CDS和氨基酸序列相似度分别为99%和98%。因此,我们认为抗源‘ARC5833’中携带的抗褐飞虱基因与Bph6为等位基因。本研究完成了籼稻品系‘ARC5833’褐飞虱抗性基因对的遗传分析与基因定位,对利用该抗源提供了科学依据。
Rice brown planthopper(BPH,Nilaparvata lugens)is one of the most serious insect pests causing rice yield reduction in China.Identification and application of BPH resistance genes is an effective way to manage the insects.Indica rice variety’ARC5833’is highly resistant to BPH.In order to clarify the resistance mechanism of indica rice’ARC5833’to BPH and to make use of it in breeding.In this study,we crossed’ARC5833’with the susceptible line’9311’to develop the F2:3 mapping population containing 145 families.Based on the seedling bulk test,109 and 36 families were respectively detected to be resistance and susceptibility in the population,which suggested one major dominant resistance gene controlling the BPH resistance phenotype.Further gene mapping indicated that one BPH resistance gene derived from’ARC5833’was mapped to one region on the long arm of chromosome 4 harbored by markers 4 M11491 and 4 M24.687.The genetic distance was 4.2 cM,during which the largest LOD score was 40 and its phenotypic variation rate was 72%.As one resistance gene Bph6 has been cloned in the same target region,the sequences of CDS and amino acid had 99%and 98%identities,respectively,according to the sequence of Bph6 and the resistance gene from’ARC5833’.Therefore,the BPH resistance gene derived from’ARC5833’was considered an allelic gene of Bph6.In this study,the genetic analysis and gene mapping of rice resistance to BPH in indica rice variety’ARC5833’was conducted,which provide scientific basis for the utilization of this resistance source.
作者
鄢柳慧
黄福钢
舒宛
张启明
赵能
莫心怡
王驰
曹凯
邱永福
Yan Liuhui;Huang Fugang;Shu Wan;Zhang Qiming;Zhao Neng;Mo Xinyi;Wang Chi;Cao Kai;Qiu Yongfu(State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Agricultural College,Guangxi University,Nanning,530005)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第18期6038-6043,共6页
Molecular Plant Breeding
基金
国家重点研发项目(2016YFD0100600)
国家自然科学基金项目(31560423)
广西自然科学基金项目(2013GXNSFGA019009)
大学生创新创业训练计划项目(201810593018)共同资助。
