摘要
目的:明确窄谱中波紫外线(narrow-band UVB,NB-UVB)对白癜风患者的黑素细胞生物学特性的影响。方法:提取34例白癜风患者皮损处黑素细胞进行体外培养。每例样本分组后分别接受不同的照射剂量(0、20、40、60、80、100 mJ/cm^2)的NB-UVB处理,照射后1 d采用免疫印迹法对黑素细胞中微管相关蛋白轻链3Ⅱ/Ⅰ(LC3Ⅱ/Ⅰ)、酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)、自噬信号磷酸化磷酸腺苷蛋白激酶(p-AMPK)、P26蛋白的表达情况进行测定。照射后2d、3d、4d采用MTT法对黑素细胞的增殖活性进行测定,采用NaOH染色法对黑素细胞中黑素含量进行测定。结果:随着NB-UVB照射剂量的增加,LC3Ⅱ/Ⅰ和p-AMPK表达水平逐渐上调,p-mTOR和P26的表达水平逐渐下降。不同NB-UVB剂量下,黑素细胞增殖水平增加,但随剂量的增加,增殖的程度逐渐减弱。不同NB-UVB剂量黑素细胞中黑素水平升高,且呈剂量依赖式递增。结论:NB-UVB能够有效调节白癜风患者黑素细胞自噬相关蛋白表达水平,活化自噬信号通路,促进黑素细胞增殖及黑素合成能力。
Objective:To determine the effect of narrow-band UVB(NB-UVB)on the biological characteristics of melanocytes from the lesions of patients with vitiligo.Methods:Melanocytes from the skin lesions of 34 patients with vitiligo were cultured in vitro.Then the melanocytes were divided into 6 groups with different radiation doses(0,20,40,60,80,100 mJ/cm^2).The levels of microtubule associated protein light chain 3Ⅱ/Ⅰ(LC3Ⅱ/Ⅰ),acidified mammalian rapamycin target protein(p-mTOR),autophagy signal phosphorylated adenosine monophosphate protein kinase(p-AMPK)and P26 protein in melanocytes were detected by Western blot at 1 day after radiation.The proliferation activity of melanocytes was measured by MTT at 2 day,3 day and 4 day after radiation.The content of melanin in melanocytes was determined by NaOH staining method.Results:The expression levels of LC3Ⅱ/Ⅰand p-AMPK increased after NB-UVB treatment in a dose-dependent manner,while the expression levels of p-mTOR and P26 decreased.The proliferation activity of melanocytes increased after treated by NB-UVB,but the degree of increase reduced gradually with the increasing doses of NB-UVB.The level of melanin increased in a dose-dependent manner.Conclusion:NB-UVB can effectively regulate the expression of autophagy-related proteins in melanocytes and activate autophagy signal pathways.At the same time,it can promote the proliferation of melanocytes and melanin.
作者
袁相凤
罗增香
刘相明
孟慧娟
李建娜
刘国艳
YUAN Xiangfeng;LUO Zengxiang;LIU Xiangming;MENG Huijuan;LI Jianna;LIU Guoyan(Department of Dermatology,Affiliated Hospital of Weifang Medical University,Weifang 261013,China)
出处
《中国麻风皮肤病杂志》
2020年第10期591-594,共4页
China Journal of Leprosy and Skin Diseases
基金
山东省医药卫生科技发展计划项目(编号:2017WS402)。
