摘要
为探究前列腺素F2α(PGF2α)信号对水牛繁殖能力的影响,利用免疫荧光染色和实时荧光定量PCR检测PGF2α受体(PTGFR)在水牛卵母细胞和胚胎早期发育过程中的表达规律;并在卵母细胞体外成熟液、胚胎培养发育液中分别添加不同浓度的PTGFR抑制剂AL-8810,检测其对水牛卵母细胞成熟效率以及胚胎发育效率的影响。结果显示,PTGFR蛋白在水牛卵母细胞及胚胎中广泛分布,在4-16细胞期胚胎阶段,PTGFR基因表达水平显著高于未受精的卵母细胞和桑椹胚;AL-8810对水牛卵母细胞核成熟效率无显著影响,但高浓度处理组(1600 nmol/L)显著降低卵母细胞早期凋亡率,100和800 nmol/L的AL-8810可显著提高体外16细胞期胚胎的发育效率,800 nmol/L组桑椹胚形成率最高。以上研究结果表明,抑制PGF2α信号可有效提高水牛卵母细胞体外成熟质量与早期胚胎发育潜力。
Buffalo has outstanding characteristics of stress resistance and adoptable to poor quality roughages and is mainly used for farming service in the world.Many of the economic traits of buffaloes have not been fully utilized and are therefore considered to be the most potential livestock for development.However,buffalo breeding methods of the world is still relatively traditional,and there are few modern scale farms,which greatly limits the development of buffalo industry and utilization of economic traits of buffalo.Compared with cattle,buffalo reproductive problems are mainly manifested in the low number of follicular reserves,insignificant estrous symptoms,low breeding efficiency,and easy loss of early embryos,which also directly limits the feeding scale of buffaloes.Therefore,with the help of modern reproductive technology(such as artificial insemination,superovulation-embryo transfer,in vitro embryo production,etc.),it is an important scientific and technological guarantee for the development of buffalo breeding industry to solve the prominent problems of buffalo reproduction in a short period of time.In vitro embryo production is an important reproductive technology,which can greatly expand the source of livestock embryos,and can improve the reproductive efficiency of livestock in a short period of time in combination with embryo transfers technology.The technical efficiency of in vitro embryo production mainly depends on in vitro oocyte maturation efficiency and early embryo development efficiency.How to optimize the methods of in vitro culture is the main focus of buffalo reproduction research.Reproductive hormones play a significant role in promoting oocyte maturation,sperm-egg interaction and embryo development and widely use in oocytes maturation and embryo culture systems,such as LH,FSH,E2 and P4.In addition,like an appropriate concentration of melatonin,vitamin C,resveratrol,sodium selenite can significantly improve the maturation quality of oocytes and promote development of embryos in vitro.Though a
作者
冉早红
何长久
张姝
刘清华
石六杰
王振
李翔
RAN Zaohong;HE Changjiu;ZHANG Shu;LIU Qinghua;SHI Liujie;WANG Zhen;LI Xiang(College of Animal Science and Technology,Huazhong Agriculture University/Animal Genetics,Breeding and Reproduction of National Center for International Research/Key Laboratory of Agriculture Animal Genetics,Breeding and Reproduction,Ministry of Education,Wuhan 430070,China)
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2020年第5期123-129,共7页
Journal of Huazhong Agricultural University
基金
国家科技重大专项(2018ZX08008-01B)
“十三五”国家重点研发计划项目(2018YFD0501605)
中央高校基础研究经费项目(2662016QD038)。
