摘要
目的调查呼伦贝尔市蒙古族已婚妇女人乳头瘤病毒(human papilloma virus,HPV)感染情况及其高危因素,探讨HPV16型病毒载量变化对宫颈上皮内瘤变I级(cervical intraepithelial neoplasia I,CIN I)患者的治疗评估的指导作用。方法利用实时荧光PCR技术对呼伦贝尔市蒙古族已婚妇女进行HPV定性筛查,使用杂交捕获二代技术检测CIN I门诊患者治疗前、治疗6个月、12个月和18个月HPV DNA含量变化情况,同时观察患者阴道分泌物及病理液基薄层细胞检测(thinprip cytologic test,TCT)检测结果的变化趋势,计量资料两组间比较利用t检验,感染高危因素采用多因素logistic回归分析统计,计数资料的分析方法为x2检验。结果蒙古族女性高危HPV感染率为22.33%(215/963),饮酒习惯、性伴侣个数是所有高危型别感染的共同高危因素。HPV16型、18型、其他高危型别感染还包括结婚次数这一高危因素。感染HPV16型的CIN I患者治疗前病毒载量与治疗后12、18个月两组间差异均有统计学意义(P<0.05),阴道清洁度镜检III度或IV度患者数及TCT检查结果显示低度不典型增生者随治疗时间延长逐渐减少,与HPV DNA含量变化一致。结论呼伦贝尔市蒙古族HPV感染率高于本地区汉族人群,需要加强蒙古族人群聚居地HPV感染相关知识的普及和宣传。高危型HPV16病毒载量的变化可作为CIN I治疗评估的观察指标。
Objective To investigate human papilloma virus(HPV)infection and the high risk factors among married women of Mongolian ethnicity in Hulunbuir area,and to explore the role of changes in HPV16 viral load in guiding the treatment evaluation of patients with cervical intraepithelial neoplasia I(CIN I).Methods Real time PCR method was used for qualitative screening of HPV in married women of Mongolian ethnicity in Hulunbeir area.hybrid capture 2 technique was used to measure HPV DNA content for CIN I patients in the outpatient at time of before treatment,and at 6,12 and 18 months after treatment.Meanwhile,the trends of vaginal secretions and the results of thin-prip cytologic test(TCT)were observed.The t-test was used to compare the measurement data of two groups.Multivariate logistic regression analysis was used for the high risk factors of infection.The chi-square test was used for the analysis of enumeration data.Results The infection rate of high risk HPV was 22.33%(215/963)in Mongolian married women in Hulunbuir area.Drinking habits and the number of sexual partners were the common high risk factors of all high-risk HPV types.The number of marriages is also the high risk factor of HPV16 and HPV18 infections.There were significant differences between the HPV DNA content in CIN I patients before treatment and the two groups at 12 and 18 months after treatment(P<0.05),respectively.The numbers of patients with degree III or IV of vaginal cleanliness under vaginoscope and with TCT results of low-grade atypical hyperplasia decreased with the treatment time.These were consistent with the change of HPV DNA content.Conclusions In Hulunbuir area,the HPV infection rate in Mongolian ethnicity was higher than that of the Han ethnicity.The popularization and propaganda of the knowledge about HPV infection in the Mongolian population settlement should be strengthened.The change of high-risk HPV DNA content can be used as the observation index for the evaluation of CIN I treatment.
作者
孙辉
孙刚
Sun Hui;Sun Gang(Department of Clinical Laboratory,Inner Mongolia Forestry General Hospital,the Second Clinical Medical School of Inner Mongolia University for Nationalities,Yakeshi 022150,China)
出处
《国际病毒学杂志》
2020年第4期329-332,共4页
International Journal of Virology
基金
2017年度内蒙古自治区卫生和计划生育科研计划项目(201703192)。
关键词
人乳头瘤病毒
蒙古族
高危因素
治疗评估
Human papilloma virus
Mongolian ethnicity
High risk factors
Treatment evaluation
