摘要
目的:体外探索性研究可口可乐和汇源橙汁及其所含饮料酸对组织蛋白酶K活性的影响,探讨饮料酸在组织蛋白酶K介导的牙本质及牙周胶原纤维降解中的可能作用。方法:本研究分为可口可乐组、汇源橙汁组、不同浓度的磷酸组(pH 2.5、pH 3.0、pH 3.5)、不同浓度的柠檬酸组(pH 2.5、pH 3.0、pH 3.5)以及阴性对照组和阳性对照组。应用明胶酶谱法,检测10种处理因素作用下组织蛋白酶K降解胶原的能力。结果:与阴性对照组相比,可口可乐处理组、汇源橙汁处理组胶原降解量升高(t=7.49、18.98,均P<0.05)。可口可乐处理组组织蛋白酶K的相对酶活性为45%;汇源橙汁处理组的相对酶活性为83%。在不同pH(2.5~3.5)的磷酸作用下,组织蛋白酶K的相对酶活性为38%~56%(t=5.69、5.21,均P<0.05)。在不同pH(2.5~3.5)的柠檬酸作用下,组织蛋白酶K的相对酶活性为41%~125%(t=4.98、20.47,均P<0.05)。结论:可口可乐和汇源橙汁能使组织蛋白酶K的活性增强,可能通过所含的磷酸或柠檬酸,促进组织蛋白酶K介导的牙本质及牙周胶原纤维降解。
Objective:To investigate the effect of Coca-Cola,Huiyuan orange juice and their beverage acid on the activity of cathepsin K in vitro.To explore the possible role of acid beverage in the degradation of dentin collagen and periodontal collagen mediated by cathepsin K.Methods:This experiment was divided into Coca-Cola group,Huiyuan orange juice group,phosphate groups of different concentrations(pH 2.5,pH 3.0,pH 3.5),citric acid groups of different concentrations(pH 2.5,pH 3.0,pH 3.5),negative control and positive control.Gelatin ZymograpHy was used to study the ability of cathepsin K to degrade collagen under the action of 10 treatment factors.Results:Compared with the control group,the collagen degradation in the Coca Cola group and Huiyuan orange juice group increased(t=7.49,18.98,all P<0.05).The relative enzyme activity of cathepsin K was 45%in Coca-Cola group and 83%in Huiyuan orange juice group.When phosphoric acid was in different pH(2.5-3.5),the relative enzyme activity of cathepsin K was 38%to 56%(t=5.69,5.21,all P<0.05).Under different pH(2.5-3.5)citric acid,the relative enzyme activity of cathepsin K was 41%to 125%(t=4.98,20.47,all P<0.05).Conclusion:Both Coca-Cola and Huiyuan orange juice can increase the activity of cathepsin K,which could accelerate cathepsin K mediated dentine and periodontal collagen degradation through the phosphoric acid or citric acid.
作者
聂海丹
王瑛慧
刘娟
刘颖
乔峰
吴丽更
NIE Hai-dan;WANG Ying-hui;LIU Juan;LIU Ying;QIAO Feng;WU Li-geng(Department of Endodontics,College of Stomatology,Tianjin Medical University,Tianjin 300070,China;Department of Stomatology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Department of Maxillofacial Surgery,College of Stomatology,Tianjin Medical University,Tianjin 300070,China)
出处
《天津医科大学学报》
2020年第5期462-465,共4页
Journal of Tianjin Medical University
