摘要
为探究不同施肥模式对土壤不同组分有机磷(Po)含量,细菌、真菌、有机磷转化相关微生物(phoD微生物)丰度和碱性磷酸酶(ALP)活性的影响。以长期施肥定位试验点土壤为试验材料,采用单因素随机区组设计,共选取不施肥(CK)、施氮钾肥(NK)、施氮磷钾肥(NPK)、单施有机肥(M)和有机无机肥配施(NPKM)5个处理。结果表明:与CK和NK处理相比,M处理土壤有机磷含量显著提高52.3%和47.8%,NPKM处理有机磷含量显著提高34.7%和30.6%。M处理土壤有效磷含量分别为CK和NK处理的34.2和31.0倍,NPKM处理有效磷含量分别为CK和NK处理的25.3和23.0倍。与CK处理比较,M和NPKM处理土壤活性Po、中度活性Po、中稳性Po和高稳性Po含量显著增加。M和NPKM处理土壤细菌、真菌、phoD微生物丰度和ALP活性均显著高于CK、NK和NPK处理。土壤活性Po、中度活性Po、中稳性Po和高稳性Po含量与phoD微生物丰度呈显著正相关(P<0.05);活性Po、中稳性Po和高稳性Po含量与ALP活性呈显著正相关(P<0.05),且不同组分有机磷受phoD微生物丰度和ALP活性的影响程度由大到小依次均为高稳性Po、活性Po、中稳性Po和中度活性Po。土壤增施有机肥增加了土壤有机磷及不同组分有机磷含量,提高了细菌、真菌、phoD微生物丰度和ALP活性。
The effects of different fertilization regimes on soil organic phosphorus fractions(Po),the abundances of bacteria,fungi,and microorganisms associated with soil organic phosphorus transformation(phoD-harboring microbial),and alkaline phosphatase(ALP)were explored by analyzing soil samples collected from a long-term fertilization experimental site.A single-factor randomized block design was adopted and five treatments were set up as follows:(1)CK,no fertilizer treatment;(2)NK,N and K fertilizer treatment;(3)NPK,N,P and K fertilizer treatment;(4)M,organic fertilizer treatment;(5)NPKM,organic and inorganic fertilizers treatment.Results show that compared with the CK and NK,soil organic phosphorus contents were significantly increased by 52.3%and 47.8%for the M,and 34.7%and 30.6%for the NPKM,respectively.The available phosphorus contents in the M was 34.2 and 31.0 times of that in the CK and NK,respectively.The available phosphorus content in the NPKM was 25.3 times and 23.0 times of that in the CK and NK,respectively.Besides,the contents of labile organic phosphorus,moderately labile organic phosphorus,moderately resistant organic phosphorus and highly resistant organic phosphorus were significantly increased for the M and NPKM,compared with the CK.In comparison with the CK,NK and NPK,organic fertilizer application(M and NPKM)significantly stimulated the abundances of bacteria,fungi,and phoD-harboring microbes,and the activity of ALP(P<0.05).In addition,soil organic phosphorus fractions were significantly and positively correlated with the phoD-harboring microbes(P<0.05).There were significant(P<0.05)positive correlations between labile Po,moderately resistant Po,highly resistant Po and ALP.The effect of phoD-harboring microbes and ALP on the organic phosphorus fractions was in the order of highly resistant Po>labile Po>moderately resistant Po>moderately labile Po.Together,the application of organic fertilizer could increase the contents of soil organic phosphorus and different organic phosphorus fractions,the abund
作者
王静
王磊
张爱君
张辉
张永春
WANG Jing;WANG Lei;ZHANG Ai-jun;ZHANG Hui;ZHANG Yong-chun(Institute of Agricultural Resources and Environments,Jiangsu Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Arable Land Conservation(Jiangsu),Ministry of Agriculture and Rural Affairs,Nanjing 210014,China;College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,China;Xuzhou Institute of Agricultural Sciences of Xuhuai District of Jiangsu Province,Xuzhou 221131,China)
出处
《生态与农村环境学报》
CAS
CSCD
北大核心
2020年第9期1161-1168,共8页
Journal of Ecology and Rural Environment
基金
江苏省自然科学基金(BK20190259)
国家甘薯产业技术体系项目(CARS-10-B9)
江苏省农业科技自主创新资金[cx(17)1001]
江苏省重点研发计划(BE2019378)
国家重点研发计划(2018YFD0200505)。
关键词
有机肥
土壤有机磷
不同组分有机磷
土壤phoD微生物
organic fertilizer
soil organic phosphorus
organic phosphorus fraction
soil phoD-harboring microbial