摘要
利用枯草芽孢杆菌制备大豆发酵液(soybean fermentation broth,S-FB)并研究了其抗氧化活性。通过分析抗氧化活性物含量得到S-FB中总酚和总肽的质量浓度比大豆发酵前提取液(soybean extract,SE)分别提高了187%和229%。抗氧化测试结果显示,1%S-FB对2,2′-联氨双-(3-乙基苯并噻唑啉-6-磺酸)[2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS]阳离子自由基的清除效果接近10%SE。因此,发酵提高了S-FB中的活性物含量并增强了其抗氧化活性。试验进一步研究了S-FB在人永生化角质形成细胞(HaCaT)内的抗氧化作用。结果表明,S-FB(5%、10%和20%)对活性氧的诱导作用随其体积分数的提高而增强。H 2O 2+S-FB(5%、10%和20%)提高的活性氧水平均低于H 2O 2或5%S-FB。因此,当活性氧水平提高,S-FB对活性氧的清除效果增强,诱导作用减弱。5%S-FB处理后,核因子E2相关因子2上调,并且超氧化歧化酶活力在36和48 h分别提高了32.5%和32.1%,对应的过氧化氢酶活力分别提高了19.6%和17.5%,同时该细胞被紫外线B损伤后的存活率是常规HaCaT细胞的1.3倍。因此,S-FB能够通过降低氧化应激反应和激活抗氧化通路来保护HaCaT细胞。
Soybean fermentation broth(S-FB)was prepared with Bacillus subtilis and its antioxidant activity was studied.Analysis of the content of active compounds revealed that the mass concentration of total polyphenols and total peptides in S-FB increased by 187%and 229%,respectively,compared with the extract of unfermented soybean(SE).The antioxidant test result suggested that the scavenging effect of 1%φ(S-FB)on ABTS(2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)cation radical was close to 10%φ(SE).Thus,fermentation increased the active compounds in S-FB and enhanced its antioxidant activity.Further research was conducted to reveal its antioxidant activity in human immortalized keratinocytes(HaCaT),which showed thatφ(S-FB)(5%,10%and 20%)induced more reactive oxygen species with the increase of its volume fraction.Moreover,H 2O 2+φ(S-FB)(5%,10%and 20%)all led to a smaller increase of reactive oxygen species than H 2O 2 or 5%φ(S-FB)did.Hence,in high reactive oxygen species(ROS)-level cells,the scavenging effect of S-FB on reactive oxygen species increased,followed by its inducting effect declined.After treatment with 5%φ(S-FB),the intracellular nuclear factor erythroid-2-related factor 2 was upregulated,and after 36 and 48 h,the superoxide dismutase activity increased by 32.5%and 32.1%,respectively,while catalase activity increased by 19.6%and 17.5%,respectively.Meanwhile,their cell viability in damage test by ultraviolet radiation B was 1.3 times higher than normal HaCaT cells.Therefore,S-FB could protect HaCaT cells by reducing the oxidative stress and activating the antioxidant pathway.
作者
陈彬和
赵炳天
孙亚娟
李云兴
CHEN Binhe;ZHAO Bingtian;SUN Yajuan;LI Yunxing(Key Laboratory of Synthetic and Biological Colloids,Ministry of Education,Jiangnan University,Wuxi 214122,China;School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2020年第17期119-124,共6页
Food and Fermentation Industries
基金
国家自然科学基金项目(51903108)
中央高校基本科研业务费专项资金项目(JUSRP11927)。
关键词
枯草芽孢杆菌
大豆发酵
抗氧化活性
人永生化角质形成细胞
活性氧
抗氧化通路
Bacillus subtilis
soybean fermentation
antioxidant activity
human immortalized keratinocytes(HaCaT)
reactive oxygen species
antioxidant pathway
