摘要
【目的】研究黑果枸杞果实在不同发育阶段基因表达谱的变化情况,为进一步开展黑果枸杞果实发育过程中的分子生物学研究提供基础资料。【方法】以黑果枸杞果实发育过程中青果期、变色期和成熟期的果实为材料,利用新一代高通量测序技术平台Illumina Solexa进行转录组测序和数据de novo组装,并对得到的Unigene进行功能注释、分类及代谢通路分析。【结果】基于转录组测序数据得到N50值为1743 bp及平均长度为1262.65 bp的黑果枸杞Unigene共有43573条。在GO、KEGG、KOG、NR和SwissProt数据库上得到注释的Unigene总数为23723条,占总Unigene的54.44%,有3726条Unigene在这5个数据库中同时得以注释,但还有19850个Unigene在这些数据库中没有得到注释。23559条和17212条Unigene分别在Nr和SwissProt数据库有同源比对信息。GO数据库中注释到的15064个Unigene分为细胞组分、分子功能及生物学过程等3大类62个功能组。KOG数据库中注释到的13128个Unigene功能系统分为25类,其中黄酮类代谢途径所属的Q类(次生代谢产物生物合成、运输和代谢)共获得了520个Unigene注释。以KEGG数据库为参考,将4951个Unigene定位到215个代谢途径分支,其中有45个Unigene与类黄酮生物合成相关。在黑果枸杞果实转录组中发现16815个SSR位点:最多的为单核苷酸SSR,占72.92%;最少的是6核苷酸,占0.04%。【结论】共得到43573条Unigene,有23723条Unigene在GO、KEGG、KOG、NR和SwissProt 5个数据库中得以注释,筛选出与类黄酮生物合成有关的Unigene 45个,这为与黑果枸杞果实品质有关基因筛选、克隆和功能分析等提供研究基础和理论依据。
【Objective】In order to sequence the transcriptome at different developmental stages of Lycium ruthenicum Murr.fruit,as well as analyze and compare the variations of their gene expression profiles,which could provide basic data for further study of molecular biology research during the development of Lycium ruthenicum fruits.【Method】The fruits of the fruiting period,the discoloration period and the maturity period of the Lycium ruthenicum were used as materials to sequence the transcriptome of Lycium ruthenicum fruit by using Illumina technology,and analyze the functional annotations and metabolic pathways of some unigene in this paper.【Result】Based on the transcriptome sequencing(RNA-Seq)data,43573 Unigene with an average length of 1262.65 bp and N50 value of 1743 bp were obtained by de novo assembly.The 23723 Unigene were annotated in GO,KEGG,KOG,NR and SwissProt databases,accounting for 54.44%,and the 3726 Unigene were annotated simultaneously in these 5 databases,but there were 19850 Unigene did not get annotated in these databases.The 23559 and 17212 Unigene were annotated in Nr and SwissProt databases,respectively.The 15064 Unigene annotated in the GO database were divided into 62 functional groups,including cellular components,molecular functions,and biological processes.The 13128 Unigene annotated in the KOG database were divided into 25 functional system categories,of which 520 Unigene annotations were obtained for the Q-class(secondary metabolite biosynthesis,transportation,and metabolism)about the flavonoid metabolic pathway.According to the KEGG database,the 4951 Unigene were mapped to 215 metabolic pathway branches,of which 45 Unigene were associated with flavonoid biosynthesis.In the transcriptome of the Lycium ruthenicum fruit,16815 SSR simple sequence repeats)loci were discovered,the majority of the loci was single nucleotide SSR,accounting for 72.92%,the minority of which was 6 nucleotides,accounting for 0.04%.【Conclusion】43573 Unigenes were obtained,23723 Unigenes were annotated i
作者
孟小伟
牛赟
马彦军
MENG Xiaowei;NIU Yun;MA Yanjun(College of Forestry,Gansu Agriculture University,Lanzhou 730070,Gansu,China;Huaiyin Normal University,Huai’an 223300,Jiangsu,China)
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2020年第9期147-155,共9页
Journal of Central South University of Forestry & Technology
基金
甘肃农业大学青年研究生指导教师扶持基金项目(GAU-QNDS-201712)
甘肃农业大学科技创新项目(林学一级学科建设开放基金)(GAU-XKJS-2018-112)
国家自然科学基金项目(31760244,31560215)。
关键词
黑果枸杞
果实发育
转录组测序
功能注释
Lycium ruthenicum
fruit ripening
transcriptome sequencing
function annotation
