摘要
目的:探讨慢性阻塞性肺疾病(COPD)并发肺炎外周血内皮祖细胞(EPC)数量及功能的变化及可能机制。方法:纳入COPD稳定期患者20例,COPD合并肺炎患者20例,正常对照组20例,通过密度梯度离心法从外周血获取单个核细胞,培养7 d后对贴壁细胞进行分析。检测EPC数量及增殖、迁移及粘附能力,一氧化氮(NO)及GTPCH(GTP环化水解酶)1/BH4通路的表达水平。结果:与正常对照组相比,COPD组外周血EPC数量减少,增殖和迁移能力显著降低,差异有统计学意义(P<0.05);与正常对照组相比,COPD并肺部感染组外周血EPC数量明显升高,而增殖、迁移粘附能力显著降低,差异有统计学意义(P<0.05);与COPD组相比,COPD并肺炎组外周血EPC增殖、迁移和粘附能力显著降低。相对对照组和COPD组,COPD合并肺炎组的GTPCH1/BH4通路表达下降,差异有统计学意义(P<0.05);此外,外周血EPC增殖、迁移和黏附能力与EPCs分泌NO水平呈正相关(R=0.65、0.79、0.70,P<0.01)。结论:COPD并发肺炎患者循环EPC功能下降与GTPCH1/BH4通路表达下降有关,EPC功能可作为评价COPD并发肺炎预测指标。
Objective:To investigate the alterations in peripheral blood endothelial progenitor cell(EPC)count and function in chronic obstructive pulmonary disease(COPD)complicated with pneumonia and its mechanism.Methods:Twenty patients with stable COPD,20 with COPD and pneumonia,and 20 normal controls were included.Peripheral blood mononuclear cells were obtained by density gradient centrifugation;and adherent cells were used for this study after 7 days of culture.The EPCs were measured for cell count,proliferation,migration,adhesion,production of nitric oxide(NO)and expression level of GTP cyclohydrolase(GTPCH)1/BH4 pathway.Results:Compared with the normal controls,the peripheral blood EPC count was lowered,and the proliferation and migration abilities were significantly reduced in stable COPD patients,with statistically significant differences(P<0.05).Compared with the normal controls,the peripheral blood EPC count was significantly increased,while the proliferation,migration and adhesion abilities were significantly decreased in COPD patients with pneumonia,with statistically significant differences(P<0.05).Compared with stable COPD group,patients with COPD and pneumonia presented significantly reductions in peripheral blood EPC proliferation,migration and adhesion abilities.Compared with the control group and stable COPD group,the GTPCH1/BH4 pathway expression in patients with COPD and pneumonia was down-regulated,with statistically significant difference(P<0.05).In addition,the peripheral blood EPC proliferation,migration and adhesion abilities were positively correlated with the level of EPC-produced NO(r=0.65,0.79 and 0.70,P<0.01).Conclusion:The lowered function of circulating EPC in COPD complicated with pneumonia is related to down-regulation of GTPCH1/BH4 pathway.EPC function can be used as a predictor of COPD complicated with pneumonia.
作者
黎银焕
张平
陈卫民
蒋鹏
Li Yinhuan;Zhang Ping;Chen Weimin;Jiang Peng(Department of Respiratory and Critical Care Medicine,Dongguan People’s Hospital,Dongguan 523059;Guangdong,Emergency Department,First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China)
出处
《广州医科大学学报》
2020年第4期50-55,共6页
Academic Journal of Guangzhou Medical University
基金
东莞市医疗卫生科技计划项目(2015105101183)。
关键词
慢性阻塞性肺疾病
肺炎
内皮祖细胞
Chronic obstructive pulmonary disease
pneumonia
endothelial progenitor cells
