摘要
激光回馈共聚焦层析成像技术因其极高的灵敏度和纵向分辨力而被应用于高分辨率的表面形貌测量、MEMS器件的内部成像,并可对生物组织内部金属异物进行定位,然而至今未曾有过对于生物组织细胞级别成像的报道。设计并搭建了基于激光回馈共聚焦层析成像技术的三维成像系统,对活体生物组织进行了探测和图像重建,能够清晰地观测到细胞的结构信息,证明了激光回馈共聚焦层析成像技术在生物组织成像领域的应用价值和发展潜力。三维成像系统可获得突破衍射极限1.4倍的横向分辨力,在使用NA=0.42的显微物镜时,横向分辨力约为1.1μm,纵向分辨力约为19.4μm,用于表面形貌测量时纵向分辨力优于500 nm。同时,设计了基于LabVIEW软件三维定位与扫描采集模块和基于Matlab软件的三维图像重建模块,可对待测区域进行精确定位,进而获得待测区域三维层析图像,具有相当的实际应用价值。
Laser feedback confocal tomography was used for high-resolution surface topography measurement,internal imaging of MEMS devices due to its extremely high sensitivity and longitudinal resolution,and can locate metal foreign objects in biological tissues.However,so far no cell-level imaging of biological tissues has ever been performed.A three-dimensional imaging system based on laser feedback confocal tomography was designed and built.The living tissue was detected and reconstructed,and the structure information of the cells can be clearly observed,which can prove its application value and development potential of technology in the field of biological tissue imaging.The three-dimensional imaging system can obtain a lateral resolution of 1.4 times the NA=0.421.1 microns and the longitudinal resolution was 19.4μm.It was used for surface topography measurement better than 500 nm.At the same time,a 3 D positioning and scanning acquisition module based on LabVIEW software and a 3 D image reconstruction module based on Matlab software were designed to accurately locate the area to be measured,and then obtain a 3 D tomographic image of the area to be measured,which has considerable practical application value.
作者
王子涵
张立
周博睿
Wang Zihan;Zhang Li;Zhou Borui(College of Science,Beijing Forestry University,Beijing 100083,China;Department of Electronic and Optical Engineering,Army Engineering University of PLA,Shijiazhuang 050051,China)
出处
《红外与激光工程》
EI
CSCD
北大核心
2020年第8期91-98,共8页
Infrared and Laser Engineering
基金
中央高校基本科研业务费专项(2015ZCQ-LY-02)。
关键词
激光移频回馈效应
共聚焦层析成像技术
三维成像
生物组织
laser frequency-shifted feedback
confocal tomography
three-dimensional imaging
biological tissue
