摘要
本试验旨在研究脂多糖(LPS)刺激对猪肠上皮细胞系(IPEC1)细胞活力、乳酸脱氢酶(LDH)活性、紧密连接蛋白和炎症相关基因表达的影响。分别选取0、0.025、1、10μg/m L的LPS刺激细胞1、2、4、8 h,检测相关指标。结果表明:在不同时间,不同浓度的LPS对细胞活力的影响未呈现出显著的规律性;与0、0.025μg/m L LPS组相比,在1、4、8h,1、10μg/mLLPS处理导致IPEC1上清液LDH活性显著升高;在不同时间,不同浓度的LPS对紧密连接蛋白(ZO-1、occludin、claudin-1)和炎症相关基因(TLR4、NF-κB、TNF-α和IL-6)mRNA表达影响未呈现出显著的规律性。以上结果表明,LPS刺激能引起IPEC1细胞损伤,但不能诱导显著的炎症反应。
The experiment was conducted to investigate the effects of lipopolysaccharide(LPS) on IPEC1 cells. The IPEC1 cells were stimulated with the different doses(0 ng/mL, 25 ng/mL, 1 μg/mL and 10 μg/mL) of LPS at different times(1, 2, 4, 8 h). The results showed that different doses of LPS had no consistent effect on cell viability at different time. LPS challenge(1 μg/mL and 10 μg/mL) increased LDH activity at 1, 4 and 8 h. Different doses of LPS had no consistent effect on mRNA expression of tight junction proteins(ZO-1, occludin and claudin-1) and inflammation related genes(TLR4, NF-κB, TNF-α and IL-6) at different time. These results indicate that LPS can cause IPEC1 cell damage, but can not result in inflammatory response.
作者
徐桥
王树辉
陈少魁
王秀英
肖勘
刘玉兰
XU Qiao;WANG Shuhui;CHEN Shaokui;WANG Xiuying;XIAO Kan;LIU Yulan(Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University,Hubei Wuhan 430023,China)
出处
《中国畜牧杂志》
CAS
北大核心
2020年第8期140-145,共6页
Chinese Journal of Animal Science
基金
武汉市应用基础前沿项目(2018020401011304)。
