摘要
目的探讨乙型肝炎病毒X蛋白(HBx)在弥漫性大B淋巴瘤(DLBCL)细胞中表达情况及对SUDHL-4细胞增殖、凋亡的影响,并探讨可能作用机制。方法通过脂质体转染方法将携带HBx基因的表达质粒转染DLBCL细胞株SUDHL-4作为SUDHL-4-HBx组,以转染空载体作为SUDHL-4-con组,未转染SUDHL-4细胞作为SUDHL-4组,通过CCK8法及荷瘤小鼠实验检测转染HBx对SUDHL-4细胞增殖的影响,通过流式细胞仪检测细胞凋亡情况,采用免疫印迹法(western blotting)检测增殖、凋亡相关蛋白表达情况。结果与SUDHL-4组、SUDHL-4-con组比较,SUDHL-4-HBx组转染后48、72、96 h OD值及裸鼠肿瘤体积显著升高(P<0.05);细胞凋亡率显著降低(P<0.05),PCNA、Bcl-2蛋白表达显著升高(P<0.05),Bax、caspase-3蛋白表达显著下降(P<0.05)。结论肝炎病毒(HBV)感染与DLBCL患者临床分期及肝损伤有关,过表达HBx可促进SUDHL-4细胞增殖,抑制其凋亡。
Objective To investigate the expression of hepatitis B virus X protein(HBx)in diffuse large B-cell lymphoma(DLBCL)and its effect on the proliferation and apoptosis of SUDHL-4 cells,and to explore possible action mechanisms.Methods The relationship between HBV infection and clinicopathological characteristics of patients was analyzed.The expression plasmid carrying HBx gene was transfected into DLBCL cell line SUDHL-4 by liposome transfection method and used as SUDHL-4-HBx group,empty vector was used as SUDHL-4-con group,and untransfected SUDHL-4 cells as SUDHL-4 group.CCK8 assay and tumor-bearing mice experiment were used to detect the effect of HBx transfection on the proliferation of SUDHL-4 cells.The apoptosis was detected by flow cytometry,and the expression of proliferation and apoptosis-related proteins was detected by western blotting.Results Compared with SUDHL-4 group and SUDHL-4-con group,the OD value and tumor volume of nude mice in SUDHL-4-HBx group were increased significantly at 48h,72h and 96h after transfection(P<0.05),and the apoptosis rate was decreased significantly(P<0.05).In addition,the expression levels of PCNA and Bcl-2 protein were significantly increased(P<0.05),however,the expression levels of Bax and caspase-3 protein were significantly decreased(P<0.05).Conclusion HBV infection is related to the clinical stage and liver injury of patients with DLBCL,and the overexpression of HBx can promote the proliferation and inhibit the apoptosis of SUDHL 4 cells.
作者
崔永哲
CUI Yongzhe(Department of Cilical Laboratory,Xincheng Hospital of Liaoyang Central Hospital,Liaoning,Liaoyang 111000,China)
出处
《河北医药》
CAS
2020年第17期2580-2584,共5页
Hebei Medical Journal
