摘要
目的:探讨宏基因二代测序(metagenomic next-generation sequencing,mNGS)技术对医院传染性疾病防控的价值。方法:回顾性分析2018年1月1日至2019年12月31日复旦大学附属中山医院收治的5例传染性疾病患者的病例资料,比较其常规微生物培养和mNGS结果。结果:5例患者常规微生物培养均为阴性,mNGS结果:炭疽芽孢杆菌1例,霍乱弧菌1例,结核分枝杆菌复合群1例,三日疟原虫1例,恶性疟原虫1例。根据mNGS结果,提早确诊传染性疾病并调整医院感染防控措施,随访30 d内,未出现院内传播新发病例。结论:mNGS在传染性疾病的早期识别与诊断中有重要作用,对于医院感染防控早期干预起到良好的指导效果。
Objective:To explore the value of metagenomic next-generation sequencing(mNGS)in the prevention and control of nosocomial infectious diseases.Methods:Retrospectively analyzed the data of 5 patients with infectious diseases from January 1,2018 to December 31,2019 in Zhongshan Hospital,Fudan University.Compared the results of mNGS and routine microbial culture.Results:Among the 5 selected patients,routine microbial culture were all negative,while the mNGS detected Bacillus anthracis,Vibrio cholerae,Mycobacterium tuberculosis complex,Plasmodium vivax and Plasmodium falciparum,respectively.All the 5 patients were diagnosed with infectious diseases earlier by mNGS,and the control measures of nosocomial infection were adjusted according to the mNGS results.No new cases of nosocomial transmission were found within 30 days of follow-up.Conclusions:The mNGS plays an important role in the early identification and diagnosis of infectious diseases,and plays a good guiding effect for the early intervention of nosocomial infection prevention and control.
作者
林佳冰
高晓东
胡必杰
陈翔
LIN Jia-bing;GAO Xiao-dong;HU Bi-jie;CHEN Xiang(Department of Hospital Infection Management, Zhongshan Hospital, Fudan University, Shanghai 200032, China;Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China)
出处
《中国临床医学》
2020年第4期554-558,共5页
Chinese Journal of Clinical Medicine
基金
上海市卫计委基金(201640313)
上海市公共卫生体系建设三年行动计划(15GWZK0101,2015)。
关键词
宏基因二代测序
医院感染预防与控制
病例系列研究
metagenomic next-generation sequencing
prevention and control of nosocomial infection
case series study
