摘要
本文以黑胡椒粉为原料,采用三相分离法(TPP)提取胡椒油树脂,研究了胡椒粉添加量、硫酸铵质量浓度、提取液与叔丁醇体积比、pH、温度对胡椒油树脂得率及胡椒碱含量的影响,并通过响应面试验优化了胡椒油树脂的提取工艺。结果表明:最佳提取条件为胡椒粉添加量5%、硫酸铵质量浓度10%、提取液与叔丁醇的体积比1∶0.5、pH 4、温度20℃,在此条件下,黑胡椒油树脂得率为12.90%,黑胡椒油树脂中胡椒碱含量为30.46%,验证试验与模型预测值基本相符。研究结果为工业化提取胡椒油树脂提供参考。
In this study,three phase partitioning(TPP)was employed to extract the pepper oleoresin from black pepper powder.The effects of the addition amount of pepper powder,ammonium sulphate concentration,ratio of crude extract to t-butanol,pH and temperature on the yield of pepper oleoresin and piperine content were studied.In addition,the extraction process of pepper oleoresin was optimized by a response surface test.The optimal condition obtained was as follow:the addition amount of pepper powder 5%,ammonium sulphate concentration 10%,ratio of crude extract to t-butanol 1∶0.5,pH 4 and temperature 20℃.Under the optimal conditions,the yield of black pepper oleoresin and piperine content was 12.90%and 30.46%,respectively.The results provide a reference for the industrial extraction of pepper oleoresin.
作者
陈星星
宋丽
谷风林
吴桂苹
陶锐
CHEN Xingxing;SONG Li;GU Fenglin;WU Guiping;TAO Rui(College of Food Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China;Spice and Beverage Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wanning,Hainan 571533,China;School of Liquor and Food Engineering/Guizhou Province Key Laboratory of Agricultural and Animal Products Processing and Storage,Guizhou University,Guiyang,Guizhou 550025,China;National Center of Important Tropical Crops Engineering and Technology Research,Wanning,Hainan 571533,China;Hainan Provincial Engineering Research Center of Tropical Spice and Beverage Crops,Wanning,Hainan 571533,China;Hainan Haiken Pepper Industry Co.,Ltd.,Haikou,Hainan 571100,China)
出处
《热带作物学报》
CSCD
北大核心
2020年第8期1674-1682,共9页
Chinese Journal of Tropical Crops
基金
海南省重大科技计划项目(No.zdkj201814)。
关键词
胡椒油树脂
三相分离法
胡椒碱
提取
pepper oleoresin
three phase partitioning
piperine
extraction
