摘要
目的对前期课题筛选出的万古霉素中介金黄色葡萄球菌(vancomycin intermediate Stapylococcus aureus,VISA)及异质性耐万古霉素金黄色葡萄球菌(heterogeneous vancomycin resistant Stapylococcus aureus,hetero-VISA)进行透射电镜下的超微结构观察。方法用KB法、琼脂稀释法和E-test法、菌谱分析法对分离出的VISA和h-VISA进行确认,并用透射电镜法对其进行超微结构观察。结果检测到的VISA和h-VISA经透射电镜观察发现菌株较标准菌株细胞壁增厚,细胞表面粗糙。结论VISA和h-VISA等万古霉素敏感性减低金黄色葡萄球菌对万古霉素耐药很可能是由于细菌细胞壁增厚所引起的。
Objective To study the ultrastructure of Vancomycin intermediate stapylococcus aureus and Heterogeneous vancomycin resistant Stapylococcus aureus isolation.Methods The resistant strains VISA and hetero-VISA were confirmed by KB method and agar dilution method and E-test and population analysis,and in the mean time studyed by transmission electron microscope.Results The VISA and h-VISA strain showed that there were thicker cell wall and rough cell surface.Conclusion Two strains of SA reduced susceptibility to vancomycin may because their thicker wall and rough cell surface.
作者
张晨
朱海平
金凤玲
孙虹佳
姚立琼
ZHANG Chen;ZHU Hai-ping;JIN Feng-ling;SUN Hong-jia;YAO Li-qiong(Department of Clinical Laboratory,the First Hospital of Lanzhou University,Lanzhou 730000,China;Department of Infection Control,the First Hospital of Lanzhou University,Lanzhou 730000,China)
出处
《现代检验医学杂志》
CAS
2020年第4期109-112,共4页
Journal of Modern Laboratory Medicine
基金
甘肃省科技支撑计划项目(项目编号:1011FKCA086)。
关键词
万古霉素耐药
万古霉素中介金黄色葡萄球菌
异质性耐万古霉素金黄色葡萄球菌
超微结构
vancomycin resistance
vancomycin intermediate Stapylococcus aureus
heterogeneous vancomycin resistant Stapylococcus aureus
ultrastructure aureus
