摘要
黄瓜花叶病毒(CMV)为辣椒的主要病害,鉴定辣椒抗CMV基因并研究其抗性机理是辣椒抗CMV育种的理论基础。前期研究发现,CA02g19570和CA02g19600为辣椒抗CMV主效QTL (qCmr2.1)的候选基因,其中CA02g19570可能性更高。本研究以辣椒抗CMV材料PBC688和易感CMV材料G29为试验材料,采用qRT-PCR和VIGS技术验证候选基因CA02g19570和CA02g19600在辣椒抗CMV中的功能。结果表明,接种CMV后0~28 d的发病过程中,抗病材料PBC688和感病材料G29中的CA02g19570基因表达量均显著上调,且抗病材料中基因上调水平显著高于感病材料;而CA02g19600在抗、感材料中的基因表达模式无显著规律。在PBC688中沉默CA02g19570后,有6个(60%)植株在接种CMV后出现严重的系统花叶症状,CA02g19570的相对表达量显著下调;而沉默CA02g19600后,仅有2个(20%)植株出现轻微的花叶症状。本研究认为CA02g19570是辣椒2号染色体上的抗CMV主效QTL (qCmr2.1)的抗性基因。
Cucumber mosaic virus(CMV) is the main disease in pepper production. Identifying the resistance genes to CMV and studying the mechanism is the basic theory of pepper resistance breeding to CMV. In the previous study, we have mapped a major QTL(qCmr2.1) underlying resistance to CMV on chromosome 2. Two candidate genes CA02 g19570 and CA02 g19600 encode the N-like proteins which play resistance to TMV. It is reasonable to postulate that CA02 g19570 is the candidate gene of qCmr2.1 for resistance to CMV in pepper. In order to verify the speculations, we analyzed the function of the candidate genes CA02 g19570 and CA02 g19600 in two pepper inbred lines, C. frutescens cv. PBC688 and C. annuum cv. G29. The results showed that the expression of CA02 g19570 was significantly up-regulated during the process of CMV infection from 0 to 28 days in CMV-resistant material PBC688 and CMV-susceptible material G29. And the up-regulated level of CA02 g19570 in PBC688 was significantly higher than that of G29. However, the CA02 g19600 did not exhibit obvious regularity in expression patterns during the process of CMV-infection in PBC688 and G29. The results showed that 6 of 10 PBC688 individuals of CA02 g19570 silencing exhibited severe systemic mosaic symptoms after inoculation with CMV while 2 of the 10 PBC688 individuals of CA02 g19600 silencing showed mild mosaic symptoms. Based on these results,CA02 g19570 was proved preliminarily to be the gene of qCmr2.1 for resistance to CMV in pepper.
作者
张根莲
郭广君
潘宝贵
刁卫平
刘金兵
戈伟
王述彬
Zhang Genlian;Guo Guang jun;Pan Baogui;Diao Weiping;Liu Jinbing;Ge Wei;Wang Shubin(College of Horticulture,Nanjing Agricultural University,Nanjing,210095;Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences,Nanjing,210014)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第17期5537-5543,共7页
Molecular Plant Breeding
基金
国家重点研发计划(2017YFD0101900)
江苏省农业重大新品种创制项目(PZCZ201714)
现代农业产业技术体系建设专项资金项目(CARS-23-G42)共同资助。
