摘要
目的:探讨不同脉冲射频(PRF)时间对坐骨神经慢性压迫性损伤(CCI)模型大鼠神经功能损伤的改善作用。方法:选择雄性健康SD大鼠,随机分为Sham组、CCI组、CCI+PRF120 s组、CCI+PRF240 s组、CCI+PRF480 s组和CCI+PRF720 s组,每组8只。CCI组、CCI+PRF120 s组、CCI+PRF240 s组、CCI+PRF480 s组、CCI+PRF720 s组均进行CCI建模处理,Sham组大鼠进行假手术处理。分别在建模后第5周开始予以CCI组、CCI+PRF120 s组、CCI+PRF240 s组、CCI+PRF480 s组、CCI+PRF720 s组分别每次0 s、120 s、240 s、480 s和720 s的PRF干预治疗。比较各组大鼠建模前1 d、建模后不同时间(1周、3周)、PRF治疗后不同时间(1 d、1周和2周)的坐骨神经功能指数(SFI)、运动神经传导速度(MNCV)、热缩足潜伏时间(TWL)和机械缩足反射阈值(MWT)以及治疗结束时PRF治疗处的坐骨神经微观结构的变化。结果:大鼠建模成功后,CCI组、CCI+PRF120 s组、CCI+PRF240 s组、CCI+PRF480 s组、CCI+PRF720 s组在1周和3周时的SFI呈明显增加趋势,MNCV、TWL和MWT呈明显降低趋势(P<0.05);Sham组大鼠建模后各指标无明显变化,仅SFI在建模后1周有明显增加。CCI+PRF120 s组、CCI+PRF240 s组、CCI+PRF480 s组、CCI+PRF720 s组大鼠经PRF干预后1 d^2周时SFI呈降低趋势,MNCV、TWL和MWT呈增加趋势。CCI+PRF480 s组、CCI+PRF720 s组大鼠经PRF干预后2周时SFI明显低于CCI+PRF120 s组、CCI+PRF240 s组,干预后1 d、1周和2周时的MNCV明显高于CCI+PRF120s组、CCI+PRF240s组,干预1周和2周时的TWL和MWT明显高于CCI+PRF120 s组、CCI+PRF240 s组(P<0.05)。CCI+PRF480 s组、CCI+PRF720 s组大鼠经PRF干预后2周时的SFI,干预后1 d、1周和2周时的MNCV以及干预后1周和2周时的MWT与Sham组比较无明显差异(P>0.05)。结论:CCI模型大鼠坐骨神经随时间延长神经功能损伤持续加重,痛阈持续下降。PRF干预能够有效改善大鼠坐骨神经损伤,具有良好的镇痛作用,每次480 s和720 s干预效果最佳。
Objective:To investigate the effect of different pulsed radio frequency(PRF)time on the improvement of nerve function in chronic compression injury(CCI)rats.Methods:Male healthy SD rats were randomly divided into sham group,CCI group,CCI+PRF120 s group,CCI+PRF240 s group,CCI+PRF480 s group,CCI+PRF720 s group,8 rats in each group.CCI modeling was performed in CCI group,CCI+PRF120 s group,CCI+PRF240 s group,CCI+PRF480 s group and CCI+PRF720 s group.Sham operation was performed in sham group.At the 5th week after modeling,PRF intervention was given to CCI group,CCI+PRF120 s group,CCI+PRF240 s group,CCI+PRF480 s group,CCI+PRF720 s group for 0 s,120 s,240 s,480 s and 720 s each time.The changes of sciatic nerve function index(SFI),motor nerve conduction velocity(MNCV),thermal with latency(TWL)and mechanical withdrawal threshold(MWT)at 1 day before modeling,at different times(1 w,3 w)before modeling,and at different time(1 d,1 w and 2 w)after PRF treatment,and the microstructure of sciatic nerve at the end of PRF treatment were compared.Results:After successful modeling,SFI of CCI group,CCI+PRF120 s group,CCI+PRF240 s group,CCI+PRF480 s group and CCI+PRF720 s group showed a significant increase trend at 1 w and 3 w,while MNCV,TWL and MWT decreased significantly(P<0.05).There was no significant change in each index in sham group,only SFI increased significantly at 1W after modeling.After PRF intervention,SFI of rats in CCI+PRF120 s group,CCI+PRF240 s group,CCI+PRF480 s group and CCI+PRF720 s group decreased from 1 d to 2 w,while MNCV,TWL and MWT increased.After PRF intervention,the SFI of rats in CCI+PRF480 s group and CCI+PRF720 s group was significantly lower than that in CCI+PRF120 s group and CCI+PRF240 s group at 2w,and the MNCV at 1D,1W and 2W was significantly higher than that in CCI+PRF120 s group and CCI+PRF240 s group at 1 d,1w and 2w,and TWL and MWT at 1 w and 2 w were significantly higher than those in CCI+PRF120 s group and CCI+PRF240 s group(P<0.05).There was no significant difference in SFI at 2 w after i
作者
孔玲
田秀娟
王世英
KONG Ling;TIAN Xiu-juan;WANG Shi-ying(Department of Pain,the Second Affiliated Hospital of Jianghan University,Wuhan 430050,Hubei,China)
出处
《川北医学院学报》
CAS
2020年第4期616-620,共5页
Journal of North Sichuan Medical College
基金
湖北省武汉市卫生计生科研基金(WX15D28)。
关键词
脉冲射频时间
神经功能
痛阈
实验研究
Pulse radiofrequency time
Nerve function
Threshold of pain
Experimental study
