摘要
目的:研究保护素D1,一种鱼油的代谢产物,对重症急性胰腺炎(SAP)小鼠肠粘膜损伤的保护作用。方法:将雄性25~30 g ICR小鼠随机分为假手术组(Sham组,行开腹关腹假手术操作)、重症胰腺炎模型组(SAP组,开腹胰管结扎建立SAP模型)和保护素D1治疗组(SAP+protectin D1组,SAP造模1 h后经眼内眦注射保护素D1,2 ng/只),每组12只,造模后24 h取材进行肠道组织病理检测及评分;免疫荧光法检测细胞凋亡、肠道上皮紧密连接蛋白闭锁小带蛋白-1(ZO-1)和Occludin表达;免疫组织化学方法检测细胞凋亡关键蛋白Caspase3表达。结果:与SAP组相比,保护素D1治疗组小鼠肠道病理损伤显著减轻。SAP组小鼠肠道上皮细胞凋亡显著增加,保护素D1治疗后肠道上皮细胞凋亡减轻,Caspase3表达下降,差异有统计学意义(P<0.01)。荧光染色检测显示,与假手术组相比,SAP模型组小鼠肠道Occludin表达显著下降,保护素D1治疗组较SAP模型组表达上升(P<0.01);SAP组小鼠肠道ZO-1表达显著下降,保护素D1治疗组较SAP组表达上升(P<0.05)。结论:保护素D1对SAP模型小鼠肠道粘膜具有保护作用,有望为SAP肠粘膜屏障功能损害提供新的治疗方法。
Objective:To study the protective effects of protectin D1,a fish oil metabolite,on the intestinal mucosa injury of mice with severe acute pancreatitis(SAP).Methods:Male 25~30 g ICR mice were randomly divided into Sham group(mice receiving sham operation for open and close abdominal surgery,n=12),SAP group(mice receiving open pancreatic duct ligation to establish SAP model,n=12)and SAP+protectin D1 group(1 hour after SAP modelling,intraocular cannula injection of protectin D1,2 ng/pc,n=12).Intestinal tissues were taken for pathological examination and scoring,immunofluorescence of apoptosis,expression of zonula occluden-1(ZO-1)and Occludin,immunohistochemistry of Caspase 3 at 24 h after modelling.Results:Compared with the SAP group,the intestinal pathological damage were significantly reduced in the SAP+protectin D1 group.The apoptosis of intestinal epithelial cells in the SAP group increased significantly.After protectin D1 treatment,the apoptosis of intestinal epithelial cells decreased and the expression of Caspase 3 decreased.The difference was statistically significant(P<0.01).Fluorescence staining showed that compared with those of the Sham group,the expression levels of Occludin in the intestine of the SAP model group were significantly reduced,and the expression levels of the SAP+protectin D1 group were higher than those of the SAP group(P<0.01).The expression level of ZO-1 in the intestine of the SAP group was significant decreased,and the expression level of ZO-1 in the SAP+protectin D1 group was higher than that of the SAP group(P<0.05).Conclusion:The protectin D1 has a protective effect on the intestinal mucosa of SAP model mice,and is expected to provide a new treatment method for restoring intestinal mucosal barrier function damage induced by SAP.
作者
李百强
武之洋
袁晨晨
马孝杰
杨琦
路国涛
李维勤
LI Bai-qiang;WU Zhi-yang;YUAN Chen-chen;MA Xiao-jie;YANG Qi;LU Guo-tao;LI Wei-qin(Jinling Hospital,Medical School of Nanjing University/General Hospital of Eastern Theater Command,Research Institute of General Surgery,Nanjing 210002,Jiangsu,China)
出处
《肠外与肠内营养》
CSCD
北大核心
2020年第4期205-210,共6页
Parenteral & Enteral Nutrition
基金
国家自然科学基金青年基金(81801970)
军队后勤科研条件实验室项目(TJJS2018002)。
关键词
保护素D1
重症急性胰腺炎
肠粘膜屏障
凋亡
Protectin D1
Severe acute pancreatitis
Intestinal mucosal barrier
Apoptosis
