摘要
目的探讨微小RNA-378a-3p(miR-378a-3p)对血管瘤内皮细胞(HemECs)增殖、侵袭、迁移的影响以及可能的作用机制。方法HemECs细胞随机分为对照组、miR-NC组及miR-378a-3p组;对照组为常规培养HemECs细胞,miR-NC组和miR-378a-3p组HemECs细胞分别在脂质体(Lipofectamine RNAIMAX)的介导下转染阴性对照以及miR-378a-3p模拟物。荧光定量PCR(qPCR)检测miR-378a-3p的表达量,细胞计数(CCK-8)检测各组细胞的增殖,Transwell实验检测细胞的侵袭、迁移;在线数据库TargetScan预测以及双荧光素酶报告基因验证miR-378a-3p与驱动蛋白超家族2A(KIF2A)的靶向关系。结果与对照组相比,miR-378a-3p模拟物能明显上调HemECs细胞中miR-378a-3p的表达量(P<0.05);上调miR-378a-3p的表达量抑制HemECs细胞增殖,降低其侵袭、迁移的能力(P<0.05);KIF2A是miR-378a-3p下游靶基因之一。结论miR-378a-3p可能通过靶向KIF2A抑制HemECs细胞的增殖、侵袭及迁移。
Objective To study the effects of microRNA-378a-3p(miR-378a-3p)on proliferation,invasion and migration of hemangioma endothelial cells(HemECs)and its possible mechanism.Methods HemECs cells were randomly divided into control group,miR-NC group,miR-378a-3p group.HemECs cells were cultured routinely in the control group.HemECs in the miR-378a-3p group were transfected with miR-378a-3p mimics by Lipofectamine RNA IMAX and miR-NC group were transfected with negative control plasmid.The expression of miR-378a-3p were detected by quantitative polymerase chain reaction(qPCR).The cells proliferation were detected by cell counting kit-8(CCK-8),and the cells invasion and migration were observed by Transwell assay.Targeting relationship between Mir 378a 3P and kinesin superfamily 2A(Kif2a)was verified by online database TargetScan prediction and double luciferase reporter gene.Results Compared with the control group,the miR-378a-3p mimic could significantly up-regulate the expression of miR-378a-3p in HemECs cells(P<0.05).Up-regulation of the expression of miR-378a-3p significantly inhibited the proliferation of HemECs cells(P<0.05),and decreased invasion and migration(P<0.05).KIF2A was one of the downstream target genes of miR-378a-3p.Conclusion miR-378a-3p may inhibit the proliferation,invasion and migration of HemECs cells by targeting KIF2A.
作者
吴卫红
潘凯
柯锦
严月华
WU Weihong;PAN Kai;KE Jin;YAN Yuehua(Department of Dermatology,China Resources WISCO General Hospital,China Resources WISCO General Hospital Affiliated to Wuhan University of Science and Technology Wuhan 430080,China;Department of Dermatology,Pudong Hospital Affiliated to Fudan University,Pudong 201399,Shanghai,China)
出处
《西部医学》
2020年第8期1128-1131,1141,共5页
Medical Journal of West China
基金
湖北省自然科学基金(2014CFB470)。
