摘要
目的探讨miR-150缺失在大气颗粒物暴露致小鼠神经炎症损伤的作用。方法 SPF级雄性C57BL/6J野生型及miR-150敲除C57BL/6J小鼠各30只,分为C57野生型清洁空气组(C57BL/6J组)、C57野生型常规空气暴露组(C57BL/6J+PM组)、miR-150敲除清洁空气组(miR-150ko组)及miR-150敲除常规空气暴露组(miR-150ko+PM组),每组15只。清洁空气组放置独立送回风净化笼具(Individual ventilation cages,IVC)饲养,常规空气组放置于常规空气饲养,实验期共12个月。应用旷场试验、新物体识别实验检测小鼠行为学变化,采用生物素双抗体夹心酶联免疫吸附法(Enzyme linked immunosorbent assay,ELISA)的方法检测小鼠海马中BDNF及炎症因子(TGF-β、IFN-γ、IL-4及IL-17)变化。结果整个实验期间常规空气中PM2.5的平均质量浓度为99.81μg/m3,IVC笼中洁净空气组PM2.5的平均质量浓度为1.24μg/m3。旷场实验结果显示,C57BL/6J+PM组及miR-150ko+PM组小鼠的总路程总运动距离、中央区域距离、边角时间、平均速度、进入中央格次数、进入中央格时间、站立次数及跨格次数均明显少于清洁空气组,miR-150ko组的边角时间、站立次数明显少于C57BL/6J组,差异有统计学意义(P<0.05)。新物体识别实验结果显示,与清洁空气组相比,C57BL/6J+PM组及miR-150ko+PM组小鼠的识别时间占总时间的百分比和识别次数占总次数的百分比均呈下降趋势,其中miR-150ko+PM组小鼠的识别时间占总时间的百分比和识别次数占总次数的百分明显少于miR-150ko组,差异有统计学意义(P<0.05)。利用ELISA法检测小鼠海马中BDNF及炎症因子结果显示,与清洁空气组相比,C57BL/6J+PM组及miR-150ko+PM组小鼠海马BDNF蛋白水平明显降低,差异有统计学意义(P<0.05)。C57BL/6J+PM组小鼠海马IFN-γ、IL-17蛋白水平明显高于C57BL/6J组,TGF-β和IL-4水平明显低于C57BL/6J组;miR-150ko+PM小鼠海马IFN-γ蛋白水平明显高于miR-150ko组,TGF-β水平明显低于miR
Objective To investigate the role of miR-150 deletion in neuroinflammatoryinduced by atmospheric particulate matter exposure in mice. Methods Thirty male C57 BL/6 J wild type and thirty miR-150 knockout C57 BL/6 J mice were divided into C57 BL/6 J wild typeclean air exposure group(C57 BL/6 J group), C57 BL/6 J wild type conventional air exposure group(C57 BL/6 J+PM group), miR-150 knockout clean air exposure group(miR-150 ko group) and miR-150 knockout conventional air exposure group(miR-150 ko+PM group), 15 in each group. The clean air exposure groups were placed in independent ventilation cages(IVC), and the conventional air exposure groups were placed in conventional air for 12 months. The behavioral changes of mice were detected by the open field test and new object recognition experiment. Enzyme linked immunosorbent assay(ELISA) was used to detect BDNF and inflammatory factors(TGF-β, IFN-γ, IL-4 and IL-17) expression levels in hippocampus of mouse. Results The average mass concentration of PM2.5 in the conventional air during the whole experiment was 99.81 μg/m3, and the average mass concentration of PM2.5 in the clean air exposure group in the IVC was 1.24 μg/m3. The result of the open field experiment showed that the total distance, the distance between the central area, the average speed, the number of times entering the central grid, the time of entering the central grid and the number of crossing of the total distance of the C57 BL/6 J+PM group and the miR-150 ko+PM groupwere all significantly less than the clean air group(P<0.05), the miR-150 ko group had significantly fewer corner time and standing times than the C57 BL/6 J group, and the difference was statistically significant(P<0.05). The result of the new object recognition experiment showed that the percentage of recognition time and the percentage of recognition times in the C57 BL/6 J+PM group and the miR-150 ko+PM group were decreased as compared with the clean air group. The percentage of recognition time and the number of recognition tim
作者
薄建柱
郑全辉
王希
李欣颖
张天琪
李爽
张艳淑
BO Jian-zhu;ZHENG Quan-hui;WANG Xi;LI Xin-ying;ZHANG Tian-qi;LI Shuang;ZHANG Yan-shu(School of Public Health,North China University of Technology,Tangshan Hebei 063210,China;School of Basic Medicine,North China University of Technology,Tangshan Hebei 063210,China;School of Life Sciences,Northwest Agricultural and Forestry University, Yangling Shanxi 712100,China;Laboratory Animal Center,North China University of Technology,Tangshan Hebei 063210,China)
出处
《毒理学杂志》
CAS
CSCD
2020年第3期203-207,213,共6页
Journal of Toxicology
基金
国家自然科学基金(81673208,31802194)
河北省高等学校科学研究计划(QN2018086,ZD2020113)
河北省中医药类科研计划课题(2018173)
唐山市科学技术研究与发展计划(19130214g)。
