摘要
目的探讨麻杏石甘汤(MGD)对重症肺炎大鼠炎症的影响及其机制。方法按照随机数字表法将正常饲养的80只大鼠分成对照组、模型组、模型+0.9%氯化钠溶液组、模型+MGD组,每组20只。对照组:正常饲养的20只大鼠;模型组:用1 mL注射器穿刺气管缓慢滴入0.3 mL新鲜配制的肺炎克雷伯菌混悬液将剩余60只大鼠进行重症肺炎模型制备;模型+0.9%氯化钠溶液组:将20只模型组大鼠进行等量的0.9%氯化钠溶液灌胃;模型+MGD组:将20只模型组大鼠进行MGD灌胃。用血气分析检测分析血气指标;酶联免疫吸附试验(ELISA)检测大鼠血清中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、白细胞介素1β(IL-1β)的含量;蛋白质印迹法检测肺组织中核因子κB抑制蛋白(IκBα)、p65、磷酸化IκBα(p-IκBα)的蛋白表达。结果成功构建肺炎克雷伯菌诱导的重症肺炎大鼠;与模型+0.9%氯化钠溶液组大鼠相比,模型+MGD组大鼠动脉血氧分压(PaO_2)[(11.89±0.83)kPa比(8.89±0.62)k Pa,P<0.05]、血氧饱和度(SaO_2)[(95.95±0.86)%比(83.05±8.62)%,P<0.05]显著升高,动脉血二氧化碳分压(PaCO_2)显著降低[(4.02±0.37)kPa比(6.49±0.59)kPa,P<0.05],肺湿干比[(4.29±0.40)%比(5.28±0.48%),P<0.05]、TNF-α[(103.24±8.42)ng/mL比(448.06±41.12)ng/mL,P<0.05]、IL-6[(85.87±8.11)pg/mL比(209.51±18.18pg/mL),P<0.05]、IL-1β[(218.54±19.33)ng/mL比(528.31±49.11)ng/mL,P<0.05]的含量均显著降低;重要的是,IκBα的表达明显上调[(1.86±0.11)比(0.25±0.02),P<0.05],p65[(0.29±0.02)比(4.10±0.04),P<0.05]、p-IκBα[(0.31±0.03)比(2.94±0.21),P<0.05]的表达明显下调。结论 MGD可抑制重症肺炎大鼠的炎症分泌,其机制可能与抑制NF-κB信号通路相关。
Objective To investigate the effect and mechanism of Maxing Shigan decoction(MGD)on inflammation of severe pneumonia in rats.Methods According to the random number table method,80 rats fed normally were divided into control group,model group,model+0.9%sodium chloride solution group,and model+MGD group,with 20 rats in each group.Control group:20 rats normally reared;Model group:Use a 1 mL syringe to puncture the trachea and slowly drop 0.3 mL of freshly prepared Klebsiel⁃la pneumoniae suspension into the remaining 60 rats for severe pneumonia model preparation;Model+0.9%sodium chloride solu⁃tion group:20 rats in the model group were given the same amount of 0.9%sodium chloride solution by gavage;model+MGD group:20 rats in the model group were given MGD by gavage.The blood gas index was analyzed.The contents of tumor necrosis factor⁃α(TNF⁃α),interleukin 6(IL⁃6)and interleukin 1β(IL⁃1β)in serum were detected by enzyme⁃linked immunosorbent assay(ELI⁃SA).The protein expressions of nuclear factor kappa B inhibitor(IκBα),p65,phosphorylated IκBα(p⁃IκBα)in lung tissue were detected by Western blot.Results The rats with severe pneumonia induced by Klebsiella pneumoniae were successfully construct⁃ed.Compared with the model+0.9%sodium chloride solution group,the arterial partial pressure of oxygen(PaO2)[(11.89±0.83)kPa vs.(8.89±0.62)kPa,P<0.05],blood oxygen saturation(SaO2)[(95.95±0.86)%vs.(83.05±8.62)%,P<0.05]in the model+MGD group increased;arterial partial pressure of carbon dioxide(PaCO2)significantly decreased[(4.02±0.37)kPa vs.(6.49±0.59)kPa,P<0.05];lung wet⁃to⁃dry ratio[(4.29±0.40)%vs.(5.28±0.48)%,P<0.05],TNF⁃α[(103.24±8.42)ng/mL vs.(448.06±41.12)ng/mL,P<0.05],IL⁃6[(85.87±8.11)pg/mL vs.(209.51±18.18)pg/mL,P<0.05],IL⁃1β[(218.54±19.33)ng/mL vs.(528.31±49.11)ng/mL,P<0.05]reduced;importantly,the expression of IκBαwas up⁃regulated[(1.86±0.11)vs.(0.25±0.02),P<0.05],while p65[(0.29±0.02)vs.(4.10±0.04),P<0.05]and p⁃IκBα[(0.31±0.03)vs.(2.94±0.21),P<0.05]was
作者
文杨
张军
王毅
WEN Yang;ZHANG Jun;WANG Yi(Emergency Medical Science Subject,Chongqing High-tech Zone People’s Hospital,Chongqing 400039,China;Respiratory Department,Chongqing High-tech Zone People’s Hospital,Chongqing 400039,China)
出处
《安徽医药》
CAS
2020年第9期1725-1728,I0003,共5页
Anhui Medical and Pharmaceutical Journal
关键词
麻杏石甘汤
肺炎
克雷伯菌感染
炎症
NF-ΚB信号通路
大鼠
Maxing Shigan decoction
Pneumonia
Klebsiella infections
Inflammation
NF⁃κB signaling pathway
Rats