摘要
目的观察桑椹对高脂喂养小鼠血糖的调节作用,为桑葚应用于血糖控制提供依据。方法50只雄性Balb/C小鼠随机分为5组;对照组(普通饲料)、高脂组(高脂饲料,脂肪占能量比60%)、低、中、高剂量桑葚组(高脂饲料基础上添加1.1%、2.2%和4.4%桑椹粉末)。每周称量体重和摄食量;实验前和实验第4、8和12周时进行腹腔葡萄糖耐量实验,计算血糖曲线下面积(AUC);13周时测定空腹血糖和胰岛素水平,计算稳态模型胰岛素抵抗指数(HOMA-IR)。结果与对照组比较,高脂组小鼠体重[(46.09±5.40)g]、空腹血糖[(11.80±1.80)mmol/L]和HOMA-IR(30.38±14.48)明显升高(P<0.05);干预13周后,与高脂组比较,4.4%桑椹组小鼠体重[(41.27±5.56)g]、空腹血糖[(9.44±1.34)mmol/L]和HOMA-IR(17.88±11.53)明显降低(P<0.05)。结论桑椹可明显降低高脂喂养小鼠体重、改善小鼠的葡萄糖耐量和胰岛素抵抗。
Objective To investigate regulative effect of mulberry on blood glucose in mice fed with high-fat diet(HFD)and to provide evidences for application of mulberry in blood glucose control.Methods Totally 50 male Balb/C mice were randomly divided into a control group(with regular chow),a HFD group(with 60%energy from fat),and three HFD plus low,moderate and high mulberry groups(with HFD and 1.1%,2.2%and 4.4%mulberry powder).Body weight and feed consumed of the mice were recorded once a week.Intraperitoneal glucose tolerance test was conducted at week 0,4,8 and 12,and area under the receiver operating characteristic curve(AUC)for blood glucose was calculated.At week 13,fasting blood glucose(FBG)and fasting insulin level were determined and homeostasis model assessment-insulin resistance(HOMA-IR)was assessed.Results Compared to the control mice,the mice of HFD group showed significantly increased body weight(46.09±5.40 g),FBG(11.80±1.80 mmol/L),and HOMA-IR(30.38±14.48)(P<0.05 for all).In comparison to those in the mice of HFD group,significantly lower body weight(41.27±5.56 g),FBG(9.44±1.34 mmol/L),and HOMA-IR(17.88±11.53)were detected one week after the 12-week treatment in the mice with 4.4%mulberry powder(all P<0.05 for all).Conclusion Mulberry intervention decreases body weight gain and improves glucose tolerance and insulin resistance in the mice fed with high-fat diet.
作者
张峥
徐加英
陈嘉平
刘伟贤
浦帅骅
李昊虬
冯昊天
秦立强
ZHANG Zheng;XU Jia-ying;CHEN Jia-ping(Department of Nutrition and Food Hygiene,School of Public Health,Soochow University,Suzhou,Jiangsu Province 215123,China;不详)
出处
《中国公共卫生》
CAS
CSCD
北大核心
2020年第8期1157-1159,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(81673101,81472974)。
