摘要
背景:骨质疏松性骨折是最常见的由骨质疏松引起的严重并发症,其修复较为困难,严重威胁患者身体健康、生活质量。目的:使用三七总皂苷对去势骨质疏松性骨折大鼠进行干预,研究相关作用机制。方法:选取50只健康雌性大鼠,10只为正常组,其余40只建立去势骨质疏松性骨折模型,分为模型组、三七总皂苷低、中、高剂量组,正常组和模型组大鼠灌胃生理盐水,三七总皂苷低、中、高剂量组大鼠分别灌胃10,20,40 mg/kg三七总皂苷溶液。实验于2019-09-23经贵州中医药大学第一附属医院动物实验伦理委员会批准,批准号为(2019)伦审第(58)号。结果与结论:三七总皂苷高剂量组大鼠骨密度、骨细胞指数均高于低、中剂量组(P<0.05)。高剂量组大鼠骨体积分数、骨痂骨小梁数量、骨小梁厚度均高于模型组、三七总皂苷低、中剂量组,骨小梁分离度低于模型组、三七总皂苷低、中剂量组(P<0.05)。三七总皂苷高剂量组大鼠谷胱甘肽过氧化物酶、骨形成发生蛋白2、血管内皮生长因子水平低于正常组,高于模型组、三七总皂苷低、中剂量组,过氧化脂、丙二醛、骨钙素、Ⅰ型前胶原羧基端前肽、骨特异性碱性磷酸酶水平高于正常组,低于模型组以及三七总皂苷低、中剂量组(P<0.05)。三七总皂苷低、中、高剂量组大鼠PI3K、Akt、mTOR表达均低于正常组、高于模型组,且三七总皂苷高剂量组大鼠PI3K、Akt、mTOR表达均高于三七总皂苷低、中剂量组(P<0.05)。提示使用三七总皂苷干预后,去势骨质疏松性骨折大鼠骨密度、骨细胞指数上升,促进骨痂处骨小梁生长,减轻大鼠氧化应激损伤,调控PI3K/Akt/mTOR信号通路,促进骨痂新生血管形成及骨折愈合。
BACKGROUND:Osteoporotic fractures are the most common serious complications caused by osteoporosis,and their repair is more difficult,which seriously threatens the health and quality of life of patients.OBJECTIVE:To investigate the interventional effect of Panax Notoginsenosides in ovariectomized osteoporotic fracture rats and the relevant mechanism.METHODS:Fifty healthy female rats were selected.Ten of them were normal group,and the other 40 rats were used to make ovariectomized osteoporotic fracture models.Model rats were divided into model group,low,medium and high dose group.Normal group and model group rats were intragastrically administered normal saline,rats in the low,medium and high dose group were given intragastric administration of 10,20 and 40 mg/kg Panax Notoginsenosides solution.The study protocol was approved by the Animal Ethic Committee of the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,No.(2019)58 on September 23,2019.RESULTS AND CONCLUSION:The bone mineral density and bone cell index of the high dose group were higher than those of low and middle dose groups(P<0.05).The bone volume fraction,the number of callus trabeculae and the thickness of trabeculae in the high dose group were all higher than those in the model group,the low dose group and the middle dose group,and the resolution of trabeculae was lower than that in these three groups(P<0.05).The levels of glutathione peroxidase,bone morphogenetic protein 2,and vascular endothelial growth factor in the high dose group were lower than those in the normal group,but higher than those in the model group,low and medium dose groups.The levels of lipid peroxide,malondialdehyde,osteocalcin,type I procollagen carboxy terminal propeptide,bone-specific alkaline phosphatase in the high dose group were higher than those in the normal group,but lower than those in the model group,low and medium dose group(P<0.05).The expression of PI3K,Akt and mTOR in the low,middle and high dose groups was lower than that in the norm
作者
胡广
关智宇
张开伟
Hu Guang;Guan Zhiyu;Zhang Kaiwei(Department of Orthopedics,the First Affiliated Hospital of Guiyang University of Traditional Chinese Medicine,Guiyang 550001,Guizhou Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2021年第2期172-177,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金项目(81960813),项目负责人:关智宇。
关键词
实验
动物
鼠
因子
蛋白
通路
骨质疏松
中药
experiment
animal
rat
factor
protein
pathway
osteoporosis
Chinese herb
