摘要
目的利用网络药理学的方法探讨钩藤治疗高热惊厥的分子作用机制。方法通过TCMSP数据库和文献挖掘获取钩藤主要活性成分,利用Perl语言分析、OMIM和GeneCards数据库预测和筛选钩藤活性成分的作用靶点。通过Cytoscape软件构建成分-靶点-通路-疾病网络。利用String数据库绘制蛋白相互作用网络。借助Bioconductor-clusterProfiler数据库,对靶点进行GO分析和KEGG通路分析。结果筛选得到钩藤27个活性成分,主要为生物碱类、萜类和黄酮类,其中生物碱类占绝大部分,涉及46个作用靶点。钩藤主要通过调节晚期糖基化产物信号通路、白细胞介素-17信号通路、低氧诱导因子-1信号通路、肿瘤坏死因子信号通路、磷脂酰肌醇信号通路等信号通路来发挥治疗高热惊厥的作用。结论钩藤中生物碱类、萜类和黄酮类等活性成分,可能是其治疗高热惊厥的物质基础,高热惊厥可能是通过调节晚期糖基化产物信号通路,白细胞介素-17信号通路,低氧诱导因子-1信号通路,肿瘤坏死因子信号通路,磷脂酰肌醇信号通路调节人体的炎症反应、细胞凋亡和调节神经元对缺氧的适应性等功能以治疗高热惊厥的。
Objective To explore the molecular mechanism of Uncaria rhynchophylla in the treatment of febrile convulsion by using network pharmacology.Methods The main active components of Uncaria rhynchophylla were obtained through TCMSP database and literature mining,and the targets of active components of Uncaria rhynchophylla were predicted and screened by Perl language analysis,OMIM and Gene Cards database.Cytoscape software was used to construct component-target-pathway-disease network.A protein interaction network was mapped using the String database.With the help of Bioconductor-cluster Profiler database,GO analysis and KEGG pathway analysis were carried out on targets.Results Totally 27 active components of Uncaria rhynchophylla were screened,mainly alkaloids,terpenes and flavonoinds,and alkaloids accounted for the most part,and 46 targets were involved.Uncaria rhynchophylla could play a role in treating FC by regulating AGE-RAGE,IL-17,HIF-1,TNF,PI3K-Akt and other signaling pathways.Conclusion The active components,such as alkaloids,terpenes and flavonoids,in Uncaria rhynchophylla may be the material basis for treating FC,and their mechanism of action involves signal pathways such as AGE-RAGE,IL-17,HIF-1,TNF,PI3K-Akt.Uncaria rhynchophylla is effective in treating FC by regulating inflammatory response,cell apoptosis and the adaptability of neurons to hypoxia.
作者
蔡小其
伦龙威
李盼
朱丽臻
石艳红
CAI Xiaoqi;LUN Longwei;LI Pan;ZHU Lizhen;SHI Yanhong(Affiliated Hospital of Traditional Chinese Medicine,Guangzhou Medical University,Guangzhou 510130,China)
出处
《中国中西医结合儿科学》
2020年第4期298-303,共6页
Chinese Pediatrics of Integrated Traditional and Western Medicine
关键词
高热惊厥
网络药理学
钩藤
分子机制
Febrile convulsion
Network pharmacology
Uncaria rhynchophylla
Molecular mechanisms
