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同位素稀释液相色谱-串联质谱法测定小麦粉中黄曲霉毒素B1的不确定度评定 被引量:5

Evaluation of the uncertainties in determination ofaflatoxin B1 in wheatmeal by liquid chromatography isotope dilution mass spectrometry
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摘要 对同位素稀释液相色谱-串联质谱法测定小麦粉中黄曲霉毒素B 1的含量进行不确定度评定,为建立有效的质量控制方法提供参考。依据CNAS—GL06《化学分析中不确定度的评估指南》和JJF 1059.1—2012《测量不确定度评定与表示》,建立数学模型,分析液相色谱-串联质谱法测定小麦粉中黄曲霉毒素B 1各个不确定度来源,并对数学模型中各不确定度分量的进行分析、量化和合成,得出黄曲霉毒素B 1的合成不确定度和扩展不确定度。当小麦粉中黄曲霉毒素B 1的含量为4.30μg/kg,扩展不确定度为0.72μg/kg(k=2)。结果表明,影响测量结果不确定度的主要因素为标准溶液的稀释配制、最小二乘法标准曲线拟合以及提取效率和免疫亲合柱的回收率。 Uncertaintyof determination of aflatoxin B 1 in wheatmeal by liquid chromatography isotope dilution mass spectrometry was evaluated to provide reference for establishing effective quality control methods.According to standards CNAS-GL 06 and JJF 1059.1—2012,a mathematical model had been established to assess the uncertainty sources in the determination of aflatoxin B 1 in wheatmeal,the uncertainty components of the mathematical model were analyzed,quantified and synthesized,the combined and expanded uncertainty were obtained.When the content of aflatoxin B 1 in wheatmeal was 4.30μg/kg,the expanded uncertainty was 0.72μg/kg(k=2).The results showed that the main factors affecting the uncertainty were calibration solution preparation,calibration curve fitting by least square method,extraction efficiency and recovery of immunoaffinity Column.
作者 杨涛 袁辉 YANG Tao;YUAN Hui(Xinjiang Product Quality Supervision and Inspection Institute,Urumqi 830011,China)
出处 《粮食与饲料工业》 CAS 2020年第3期7-11,共5页 Cereal & Feed Industry
关键词 同位素稀释液相色谱-串联质谱法 小麦粉 黄曲霉毒素B 1 不确定度 isotope dilution liquid chromatography-tandem mass spectrometry wheatmeal aflatoxin B 1 uncertainty
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