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一种国产龋活性培养基与标准培养基一致性及有效性的实验检测 被引量:1

A test of consistency and availability of domestic caries active medium and standard caries active medium
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摘要 目的比较一种国产龋活性培养基和日本Cariostat龋活性培养基在细菌产酸性方面的一致性、敏感性和准确性,借此评估国产龋活性培养基的临床可行性。方法对变异链球菌、血链球菌、唾液链球菌、远缘链球菌、嗜酸乳杆菌、牙龈卟啉单胞菌、具核梭杆菌、黏性放线菌8种测试菌种4个浓度梯度以及空白对照样本,采用两种培养基分别培养48 h,相同条件下,采用单盲法分别由3位检查者进行读数,并采用SPSS 19.0统计软件对结果进行统计分析。结果两种培养基Kappa值大于0.8,国产试剂盒相对于日本试剂盒的敏感度为94.7%,特异度为85.6%,准确率为90.4%。结论本次实验选择的国产菌斑产酸检测技术可能是一种非常有希望的替代品,龋病为多因素致病疾病,仍需要寻求更多高准确度、高性价比、易获取的实验产品。 Objective To compare the consistency,sensitivity and accuracy between a caries activity test from China and Cariostat test from Japan and to evaluate the clinical feasibility of the former one.Methods Eight species of oral bacteria,including S.mutans,S.sanguinis,S.salivarius,S.sobrinus,L.acidophilus,P.gingivalis,F.nucleatum and A.viscosus,were diluted in four concentrations and cultured in these two mediums separately for 48 hours.Blank negative control was also applied.Three blind tester reported the results under same condition,and the results were then analyzed by SPSS 19.0.Results The kappa values of these two tests were over 0.8.Compared with the one from Japan,the sensibility of the domestic test kit was 94.7%,specificity was 85.6%and accuracy was 90.4%.Conclusion The acid-production detection technique from oral plaque selected in this study is a promising substitute for Cariostat test.Caries is a disease with various contributing factors,so more experimental products with better accuracy,higher cost performance and easier accessibility are still needed.
作者 VIVIEN FENG 张羽 曹桂芝 吴日玥 陈曦 FENG Vivien;ZHANG Yu;CAO Guizhi;WU Riyue;CHEN Xi(Department of Preventive Dentistry,Shanghai Ninth People's Hospital,College of Stomatology,Shanghai Jiao Tong University School of Medicine/National Clinical Research Center for Oral Diseases/Shanghai Key Uiboratory of Stomatology&Shdiighai Research Institute of Stomatology,Shanghai 200011,China)
出处 《口腔医学》 CAS 2020年第7期602-605,共4页 Stomatology
基金 国家自然科学基金(81800967) 上海市卫健委适宜技术项目(2019SY027)。
关键词 龋病 龋活性测试 Cariostat 国产培养基 caries caries activity test Cariostat domestic medium
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