摘要
The manipulation of physicochemical and biological properties of the encapsulated cells by cell surface engineering technique is now attracting more and more researchers’interest.In this study,we showed a mild and highly efficient way to build a type of polymer-living cell conjugates based on photoinduced electron transfer-reversible addition-fragmentation chain-transfer polymerization(PET-RAFT).The polymerization could proceed at room temperature under the blue light initiating and showed very less influence on the activity of the living cells.Significantly,the constructed positively charged polymer-living cell conjugates could aggregate with the negatively charged native Chlorella pyrenoidosa(CP)cell to form a microscale aggregate which then allowed a functionality switching of the encapsulated CP cells from the normal photosynthetic O2 towards H2 production with an average rate of 0.26μmol H2h-^1(mg chlorophyll)-^1 for 42 h.It is then anticipated that the method we show could contribute an effective way for generating different functionalized living cell-polymer hybrid structures on demand,and the demonstrated aggregation-induced functionality switching of the CP cells is also anticipated to inspire more applications of the cell-based materials towards biology green energy.
基金
supported by the National Natural Science Foundation of China(Grant Nos.51873050,21871069 and 51703043)
the China Postdoctoral Science Foundation(Grant No.2015M571401)。
