摘要
为诊断和防治红松(Pinus koraiensis)根腐病,从黑龙江省黑河市和伊春市苗圃地收集红松病根,采用组织分离法得到纯培养物后接种到健康的红松上,测定其致病性;通过形态观察和分子生物学鉴定其种类,并利用PCR技术对发病组织和健康组织中的病原菌进行了特异性检测。结果表明:从病变组织中分离出1种真菌,人工培养的菌落初期为白色棉絮状,随后变成浅红色。气生菌丝上产孢,孢子着生于长筒形单瓶梗上,分生孢子镰刀形或月牙型。根据ITS和TEF-1α基因测序比对结果并结合形态特征将该菌鉴定为尖孢镰刀菌(Fusarium oxysporum)。将该菌接种于健康红松,发病症状与苗圃地红松发病症状相同。此外,从红松苗圃地土壤中能够检测到尖孢镰刀菌,这一现象表明土壤可能是该病的传播方式。
The Pinus koraiensis Sieb.et Zucc.root rot pathogens were isolated and identified in the cities of Heihe and Yichun of Heilongjiang Province.Their pathogenicity and source of infection were analyzed.After disinfecting the surface of the diseased root tissue collected and inoculate onto PDA medium.The resulting pure cultures were inoculated onto a healthy host for determination their pathogenicity.Pathogens morphological characteristics were observed by optical microscope and SEM.Pathogens were identified by ITS and TEF-1αgenes.PCR were used to detect if there were Fusarium oxysporum in diseased and healthy tissues.The result shows that strain was isolated from the diseased tissue.In the beginning,it was a white cotton-like colony.While colony color turn to light red,there was a purple-red pigment on the back.Sporulation on aerial hyphae,spores are born in long cylindrical single-bottles,conidia sickle-shaped,crescent-shaped,and slightly curved.The strain was identified as F.oxysporum by molecular identification and its morphological characteristics.The pathogenicity test was carried out by inoculating F.oxysporum on healthy P.koraiensis,the symptoms of the disease are same to those on the original nursery.In addition,F.oxysporum can be detected from the nursery soil.This phenomenon indicates that the soil is likely to be the major transmission mode of pathogens.
作者
张平
刁健
王立海
Zhang Ping;Diao Jian;Wang Lihai(Northeast Forestry University Harbin 150040,P.R.China)
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2020年第7期123-127,共5页
Journal of Northeast Forestry University
基金
国家自然科学基金项目(31570547)
“双一流”创新人才培养专项项目(000/41113102)。
关键词
病原鉴定
尖孢镰刀菌
红松
根腐病
Pathogen identification
Fusarium oxysporum
Pinus koraiensis
Root rot disease
