摘要
以构树(Broussonetia papyrifera)组培生根苗为试材,研究了50、100和150 mmol/L NaCl胁迫对质膜总固醇、质膜微囊脂肪酸组成及膜流动性的影响。结果表明:50和100 mmol/L NaCl处理导致叶片中质膜总固醇摩尔质量浓度升高,150 mmol/L NaCl处理后固醇摩尔质量浓度与对照组无差异,而根中质膜总固醇摩尔质量浓度变化不显著。构树幼苗根部质膜以饱和脂肪酸为主,其中棕榈酸(C16:0)摩尔分数最高,占52.75%;其次为硬脂酸(C18:0)、山萮酸(C22:0)等。不饱和脂肪酸有棕榈油酸(C16:1)、反油酸(C18:1n9t)、油酸(C18:1n9c)、花生四烯酸(C20:4n6)等。与根中不同,叶片质膜中的脂肪酸以不饱和脂肪酸居多,如棕榈油酸(C16:1)、反油酸(C18:1n9t)、油酸(C18:1n9c)和花生四烯酸(C20:4n6),饱和脂肪酸仅有棕榈酸(C16:0)和硬脂酸(C18:0)两种。NaCl处理后,根部和叶片脂肪酸各组分配比发生了明显变化。根中饱和脂肪酸摩尔分数增加,不饱和脂肪酸摩尔分数降低。而叶中反油酸(C18:1n9t)和硬脂酸(C18:0)摩尔分数显著升高。与根中相似,叶片质膜脂肪酸不饱和指数(IUFA)在NaCl胁迫后降低,同时根部和叶片质膜流动性均随之下降。总之,高浓度NaCl(150 mmol/L)处理引起构树质膜脂肪酸组成变化,表现为脂肪酸饱和度增加和质膜流动性下降。质膜膜脂对盐胁迫环境的响应研究,可为质膜参与植物耐盐性研究提供新的思路。
Broussonetia papyrifera rooted plantlets were treated with 50, 100 and 150 mmol/L NaCl and the effect of salt stress on plasma membrane (PM) total sterol, lipid composition and membrane fluidity was investigated. The total sterol content in the leaves increased under 50 and 100 mmol/L NaCl, while 150 mmol/L NaCl had no effect on sterol content as compared with control. In contrast, the content of sterol in the roots changed unobvious. The plasma membrane isolated from the roots was mainly composed of saturated fatty acids among which palmitic acid (C16:0) was the main component, which accounted for 52.75% of the total fatty acids. And the other saturated fatty acids included stearic acid (C18:0), and eicosanoic acid (C20:0). The unsaturated fatty acids in root PM included palmitoleic acid (C16:1), elaidic acid (C18:1n9t), oleinic acid (C18:1n9c), arachidonic acid (C20:4n6) and so on. In contrast to the roots, the lipid composition of leaf PM was primarily comprised of unsaturated fatty acids, including palmitoleic acid (C16:1), elaidic acid (C18:1n9t), oleinic acid (C18:1n9c) and arachidonic acid (C20:4n6). However, it contained only two saturated fatty acids, palmitic acid (C16:0) and stearic acid (C18:0), in the PM of the leaves. After NaCl treatment, obvious changes in the composition of the lipids in the PM were detected both in the roots and leaves. In the roots, the contents of the saturated fatty acids increased, whereas, the concentration of the unsaturated fatty acids declined. The fatty acids elaidic acid (C18:1n9t) and stearic acid (C18:0) increased significantly in the leaves. Like the roots, the index of unsaturated fatty acids (IUFA) of the plasma membrane purified from NaCl stressed leaves declined, meanwhile, the fluidity of the PM was declined both in the roots and leaves in response to NaCl treatment. Therefore, the main lipid change induced by high concentration NaCl (150 mmol/L) in the plasma membrane was an increase in the degree of saturation and a decline in the membrane fluidity. The inves
作者
张强
黄婧
周鹏
张敏
Zhang Qiang;Huang Jing;Zhou Peng;Zhang Min(Nanjing Forestry University,Nanjing 210037,P.R.China;Jiangsu Academy of Forestry)
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2020年第8期22-26,49,共6页
Journal of Northeast Forestry University
基金
江苏省林业科技创新与推广项目(LYKJ[2018]04)
江苏省高校优秀中青年教师和校长境外研修计划(苏教办师[2018]3号)
关键词
构树
NACL胁迫
质膜
固醇摩尔质量浓度
脂肪酸组成
膜流动性
Broussonetia papyrifera
NaCl stress
Plasma membrane
Sterol content
Lipid composition
Membrane fluidity
